Autophagy is constitutively active in normal mouse sino-atrial nodal cells.
Autophagy is constitutively active in normal mouse sino-atrial nodal cells.
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DOI：
10.1267/ahc.11030
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发表时间：
2011-10-26
影响因子：
2.4
通讯作者：
Matsuura H
中科院分区：
文献类型：
--
作者：
Omatsu-Kanbe M;Yamamoto T;Matsuura H
本研究旨在观察正常成年小鼠心脏窦房结细胞的自噬现象。自噬是负责通过溶酶体消化降解和回收长寿命和/或受损细胞质组分的细胞过程。在心脏中，已知自噬在生理条件下以低水平发生，但当细胞暴露于某些应激（如缺血）时，自噬会上调。我们研究了SA结细胞自噬的基础水平是否与心室或心房肌细胞不同。超微结构分析表明，SA结细胞含有一定数量的自噬空泡（自噬体）与溶酶体消化降解的各个阶段，而在心室或心房肌细胞的数量是可以忽略不计或非常小。自噬体标记物微管相关蛋白1轻链3（LC 3）和溶酶体标记物溶酶体相关膜蛋白1（LAMP 1）的免疫组化染色显示SA结细胞中自噬体和溶酶体的含量均明显高于普通心肌细胞。我们的研究结果提供了证据表明，自噬是活跃的正常SA结细胞，这不是一个应激激活的过程，但在小鼠心脏的组成性事件。
This study was designed to examine the autophagy in sino-atrial (SA) nodal cells from the normal adult mouse heart. Autophagy is the cellular process responsible for the degradation and recycling of long-lived and/or damaged cytoplasmic components by lysosomal digestion. In the heart, autophagy is known to occur at a low level under physiological conditions, but to become upregulated when cells are exposed to certain stresses, such as ischemia. We examined whether the basal level of autophagy in SA nodal cells was different from that in ventricular or atrial myocytes. An ultrastructural analysis revealed that the SA nodal cells contained a number of autophagic vacuoles (autophagosomes) with various stages of degradation by lysosomal digestion, whereas the number of those in ventricular or atrial myocytes was either negligible or very small. The immunostaining of autophagosome marker microtubule-associated protein 1 light chain 3 (LC3) and lysosome marker lysosome-associated membrane protein 1 (LAMP1) indicated that the content of both autophagosomes and lysosomes were much greater in SA nodal cells than in ordinary cardiomyocytes. Our results provide evidence that the autophagy is active in normal SA nodal cells, which is not a stress-activated process but a constitutive event in the mouse heart.
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影响因子：
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作者：
CHEN, JW;MURPHY, TL;WILLINGHAM, MC;PASTAN, I;AUGUST, JT
通讯作者：
AUGUST, JT
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通讯作者：
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