Antigenic and genetic characterization of the fusion (F) protein of mumps virus strains
Antigenic and genetic characterization of the fusion (F) protein of mumps virus strains
复制标题
流行性腮腺炎病毒株融合（F）蛋白的抗原和遗传特征
作者：
T. Tecle;B. Johansson;Z. Yun;C. Örvell
对12株流行性腮腺炎病毒进行了融合蛋白基因的核苷酸序列分析，它们属于小分子疏水蛋白基因的A、C和D三种类型。将该基因的核苷酸序列和推导的氨基酸序列进行比对，并与以前报道的序列进行比较。此外，用10株针对A型F蛋白的单抗对不同A、C和D型毒株的F蛋白进行了抗原性比较。对F基因编码区的系统发育分析表明，与先前根据SH蛋白基因确定的不同的基因类型一样，F基因的编码区具有预期的聚集性。对该蛋白的538个氨基酸序列的比较表明，不同毒株之间只有少量氨基酸存在差异。A型与B型、C型和D型的氨基酸差异较小，而B、C和D型之间的一致氨基酸差异较小。10株单抗中有9株与C型和D型反应，1株不与C型和D型反应。结果表明，F蛋白的结构和抗原性在基因内和基因间长期保存良好。
Summary. Twelve strains of mumps virus, belonging to the A, C and D genotypes of the small hydrophobic (SH) protein gene, were investigated by nucleotide sequencing of the fusion protein gene. The nucleotide sequences and deduced amino acid sequences were aligned and compared with previously reported sequences of the gene. In addition an antigenic comparison between the F protein of different strains of the A, C and D genotypes was performed with ten monoclonal antibodies directed against the F protein of genotype A. Phylogenetic analysis of the coding region of the F gene showed the expected clustering of the different genotypes, as previously determined from the SH protein gene. Comparison of the 538 long amino acid sequence of the protein showed that only a small number of amino acids differed between the viral strains. The A genotype differed from B, C and D whereas the latter showed fewer consistent amino acid differences between themselves. Nine of the ten monoclonal antibodies reacted with the C and D genotypes and one failed to react with these genotypes. It is concluded that the structure and antigenicity of the F protein is well conserved both intra- and intergenotypically over long periods of time.
腮腺炎病毒融合蛋白基因的克隆和测序。
影响因子：
3.7
作者：
Waxham,MN;Server,AC;Goodman,HM;Wolinsky,JS
通讯作者：
Wolinsky,JS