Correlation between intraluminal oxygen gradient and radial partitioning of intestinal microbiota.
Correlation between intraluminal oxygen gradient and radial partitioning of intestinal microbiota.
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DOI：
10.1053/j.gastro.2014.07.020
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发表时间：
2014-11
期刊：
影响因子：
29.4
通讯作者：
Wu GD
中科院分区：
文献类型：
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作者：
Albenberg L;Esipova TV;Judge CP;Bittinger K;Chen J;Laughlin A;Grunberg S;Baldassano RN;Lewis JD;Li H;Thom SR;Bushman FD;Vinogradov SA;Wu GD
肠道菌群是由宿主生理决定的动态环境中一个复杂而密集的群落。我们研究了肠道氧水平如何影响粪便和粘膜粘附微生物群的组成。我们使用磷光猝灭法和专门设计的腔内氧探针来动态量化小鼠肠道腔内氧水平。使用16S rRNA基因测序来表征暴露于高压氧的小鼠肠道、人类直肠活检和粘膜拭子样本以及配对的人类粪便样本中的微生物群。盲肠管腔的平均pO2值极低（<1 mmHg）。在改变小鼠肠道氧合的过程中，我们观察到氧气从肠道组织扩散，并建立了一个从组织界面延伸到管腔的径向梯度。用高压氧增加组织氧合改变了小鼠肠道微生物群的组成。在人类中，16S rRNA基因分析显示，与粪便相比，与直肠粘膜相关的变形菌门和放线菌门耐氧生物的比例增加，表明氧合对微生物群的影响。厚壁菌门和拟杆菌门的一种主要代谢蛋白胨和氨基酸的无糖细菌联合体，主要与粘液有关。这可能是由于粘液提供的蛋白底物的存在和肠上皮的脱落。在对小鼠和人类肠道微生物群的分析中，我们观察到微生物的径向梯度与宿主组织提供的氧气和营养物质的分布有关。
The gut microbiota is a complex and densely populated community in a dynamic environment determined by host physiology. We investigated how intestinal oxygen levels affect the composition of the fecal and mucosally adherent microbiota. We used the phosphorescence quenching method and a specially designed intraluminal oxygen probe to dynamically quantify gut luminal oxygen levels in mice. 16S rRNA gene sequencing was used to characterize the microbiota in intestines of mice exposed to hyperbaric oxygen, human rectal biopsy and mucosal swab samples, and paired human stool samples. Average pO2 values in the lumen of the cecum were extremely low (<1 mmHg). In altering oxygenation of intestines of mice, we observed that oxygen diffused from intestinal tissue and established a radial gradient the extended from the tissue interface into the lumen. Increasing tissue oxygenation with hyperbaric oxygen altered the composition of the gut microbioita in mice. In humans, 16S rRNA gene analyses revealed an increased proportion of oxygen-tolerant organisms of the Proteobacteria and Actinobacteria phyla associated with the rectal mucosa, compared with the feces, indicating an effect of oxygenation on the microbiota. A consortium of asaccharolytic bacteria of the Firmicute and Bacteroidetes phyla, which primarily metabolize peptones and amino acids, was associated primarily with mucus. This could be due to the presence of proteinaceous substrates provided by mucus and the shedding of the intestinal epithelium. In an analysis of intestinal microbiota of mice and humans, we observed a radial gradient of microbes linked to distribution of oxygen and nutrients provided by host tissue.
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影响因子：
2.9
作者：
Mani, Tomoyasu;Niedzwiedzki, Dariusz M.;Vinogradov, Sergei A.
通讯作者：
Vinogradov, Sergei A.
聚糖代谢如何塑造人类肠道微生物群。
DOI：
10.1038/nrmicro2746
发表时间：
2012-04-11
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
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影响因子：
4.4
作者：
BRUNE, A;EMERSON, D;BREZNAK, JA
通讯作者：
BREZNAK, JA
DOI：
10.1099/00207713-51-4-1521
发表时间：
2001-07-01
影响因子：
2.8
作者：
Ezaki, T;Kawamura, Y;Shu, SE
通讯作者：
Shu, SE
影响因子：
64.8
作者：
通讯作者：
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