Isolation from wheat mitochondria of a membrane-associated high molecular weight complex involved in DNA synthesis
Isolation from wheat mitochondria of a membrane-associated high molecular weight complex involved in DNA synthesis
复制标题
从小麦线粒体中分离参与 DNA 合成的膜相关高分子量复合物
DOI：
10.1007/bf00020561
复制
发表时间：
1991
影响因子：
5.1
通讯作者：
S. Litvak
中科院分区：
文献类型：
--
作者：
M. Echeverria;D. Robert;J. Carde;S. Litvak
用蔗糖梯度离心从纯化的小麦胚线粒体中分离出一个与线粒体膜相关的高分子量线粒体DNA （mtDNA）复制复合体。该复合体包括mtDNA以及参与细胞器基因组复制和转录的酶活性，如DNA聚合酶、RNA聚合酶和i型拓扑异构酶。分离的复合体在体外mtDNA和mtRNA合成中具有活性。电镜和脂质分析证实了这种复合物的膜起源。酶活性对生理离子强度的抗性为0.1-0.2 M KCl，而膜- mtdna结合的抗性可达1 M KCl。DNA酶处理释放了DNA聚合酶活性，而蛋白酶处理溶解了mtDNA，表明mtDNA与膜蛋白直接相互作用。使用一种新的方法来检测线粒体膜在Sal I消化后特异性保留的mtDNA片段，表明特定的mtDNA序列将mtDNA锚定在线粒体膜上。
A high molecular weight mitochondrial DNA (mtDNA) replication complex, associated with the mitochondrial membrane, was isolated by sucrose gradient centrifugation from purified wheat embryo mitochondria. This complex comprised the mtDNA as well as enzyme activities involved in the replication and transcription of the organelle genome, such as DNA polymerase, RNA polymerase and topoisomerase type I. The isolated complex is active in mtDNA and mtRNA synthesis in vitro. Electron microscopy and lipid analysis confirmed the membrane origin of this complex. Enzyme activities are resistant to physiological ionic strengths, 0.1–0.2 M KCl, while the membrane-mtDNA association is resistant up to 1 M KCl. DNase treatment of the complex released the DNA polymerase activity while protease treatment solubilized mtDNA, suggesting the direct interaction of mtDNA with membrane protein(s). The use of a novel approach to detect mtDNA fragments specifically retained by the mitochondrial membranes after Sal I digestion of the complex suggests that specific mtDNA sequences anchor mtDNA to mitochondrial membranes.
影响因子：
11.4
作者：
CHANG, DD;HAUSWIRTH, WW;CLAYTON, DA
通讯作者：
CLAYTON, DA
膜附着激活 dnaA 蛋白，这是大肠杆菌中染色体复制的起始蛋白。
DOI：
10.1073/pnas.85.19.7202
发表时间：
1988
影响因子：
11.1
作者：
Yung,BY;Kornberg,A
通讯作者：
Kornberg,A