Suppression of natural killer cell activity in harbour seals (Phoca vitulina) fed Baltic Sea herring
Suppression of natural killer cell activity in harbour seals (Phoca vitulina) fed Baltic Sea herring
复制标题
喂养波罗的海鲱鱼的斑海豹 (Phoca vitulina) 自然杀伤细胞活性的抑制
DOI：
10.1016/0166-445x(95)00031-x
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发表时间：
1996
影响因子：
4.5
通讯作者：
A. Osterhaus
中科院分区：
文献类型：
--
作者：
Peter S. Ross;R. L. Swart;H. Timmerman;P.J.H. Reijnders;J. G. Vos;H. Loveren;A. Osterhaus
近年来，海洋哺乳动物种群的大规模死亡引发了人们的疑问，即通过海洋食物链积累的污染物可能起到了促进作用。虽然病毒被证明是暴发的主要原因，但不能排除受影响动物中有机氯化学物质的免疫毒性作用。我们进行了为期两年半的免疫毒理学实验，其中两组海豹各11只，分别来自相对受污染的波罗的海和相对未受污染的大西洋。两年后，波罗的海海豹在鲸脂中积累的阿受体介导的2，3，7，8-TCDD毒性当量水平是大西洋海豹的3-4倍。在实验过程中，总共采集了17次血液进行免疫学评估，在此期间，从摄食波罗的海鲱鱼的海豹中分离出的外周血单个核细胞的自然细胞毒活性下降到比摄食大西洋鲱鱼的海豹低约25%的水平(P<；0.01)。自然杀伤(NK)细胞活性以前没有被描述为海洋哺乳动物物种。我们鉴定了海豹外周血单个核细胞(PBMC)的天然细胞毒活性，发现这是白介素2(IL-2)反应，对抗asialo GM1抗体敏感，对病毒感染的靶细胞(如其他哺乳动物的NK细胞)具有更高的杀伤活性。由于NK细胞是白细胞，在抵御病毒的第一道防线中发挥着重要作用，观察到以波罗的海鲱鱼为食的海豹的NK细胞活性受损，表明暴露在污染物中可能对欧洲受污染水域的海豹抵御病毒感染产生不利影响。因此，这可能影响了最近疫情期间感染的严重程度、存活率和感染的传播。
Mass mortalities among marine mammal populations in recent years have raised questions about a possible contributory role of contaminants accumulated through the marine food chain. While viruses were shown to be the primary cause of the outbreaks, an immunotoxic action by organochlorine chemicals in affected animals could not be ruled out. We carried out a 2 1 2 - year immunotoxicological experiment in which two groups of 11 harbour seals each were fed herring from either the relatively contaminated Baltic Sea or the relatively uncontaminated Atlantic Ocean. Seals in the Baltic Sea group accumulated 3–4 times higher levels of Ah-receptor-mediated 2,3,7,8-TCDD Toxic Equivalents in blubber than did their Atlantic counterparts following 2 years on the respective diets. Blood was sampled a total of 17 times during the course of the experiment for immunological evaluation, during which time the natural cytotoxic activity of peripheral blood mononuclear cells isolated from seals fed Baltic Sea herring declined to a level approximately 25% lower than that observed in seals fed Atlantic herring (P < 0.01). Natural killer (NK) cell activity has not been previously described for a marine mammal species. We characterized the natural cytotoxic activity of harbour seal peripheral blood mononuclear cells (PBMC), and found this to be interleukin-2 (IL-2) responsive, sensitive to antibody anti-asialo GM1, and it was higher against a virus-infected target cell, like NK cells described for other mammals. As NK cells are leukocytes which play an important role in the first line of defence against viruses, the observed impairment of NK cell activity in the seals feeding on the Baltic Sea herring suggests that exposure to contaminants may have an adverse effect on the defence against virus infections in seals inhabiting polluted waters in Europe. This may therefore have affected the severity of the infections, the survival rates and the spread of infections during recent epizootics.
NC 和 NK 效应子增强 1 型单纯疱疹病毒感染小鼠细胞系的裂解作用。
DOI：
--
发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Colmenares,C;Lopez,C
通讯作者：
Lopez,C
干扰素无法保护病毒感染的细胞免受自然杀伤 (NK) 细胞的裂解，这与 NK 细胞介导的体内抗病毒作用相关。
DOI：
--
发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Bukowski,JF;Welsh,RM
通讯作者：
Welsh,RM
影响因子：
158.5
作者：
BIRON, CA;BYRON, KS;SULLIVAN, JL
通讯作者：
SULLIVAN, JL