Genome-wide screening of the genes required for tolerance to vanillin, which is a potential inhibitor of bioethanol fermentation, in Saccharomyces cerevisiae.
Genome-wide screening of the genes required for tolerance to vanillin, which is a potential inhibitor of bioethanol fermentation, in Saccharomyces cerevisiae.
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DOI：
10.1186/1754-6834-1-3
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发表时间：
2008-04-15
影响因子：
6.3
通讯作者：
Shima J
中科院分区：
文献类型：
--
作者：
Endo A;Nakamura T;Ando A;Tokuyasu K;Shima J
木质纤维素材料丰富，是生物乙醇生产最重要的潜在来源之一。尽管木质纤维素的预处理对于有效的糖化和发酵是必要的，但在预处理步骤中会产生许多副产物，包括呋喃衍生物、弱酸和酚类化合物。许多这些成分抑制酵母的生长和发酵。特别是，香兰素是木质纤维素水解物中最有效的抑制剂之一，因为它在非常低的浓度下抑制发酵。为了鉴定对香兰素耐受所需的基因，我们筛选了一组二倍体酵母缺失突变体，这是阐明特定基因功能的有力工具。76个缺失突变体被鉴定为香草素敏感突变体。在香兰素敏感突变体中，大量缺失的基因被归类为“染色质重塑”和“囊泡运输”的功能类别，这表明这些功能对香兰素耐受性很重要。对香草素敏感突变体对呋喃衍生物、弱酸和酚类化合物的交叉敏感性也进行了研究。麦角甾醇生物合成基因是对所有抑制化合物耐受性所必需的，这表明麦角甾醇是对各种抑制剂耐受性的关键成分。我们的分析预测，酿酒酵母的香兰素耐受性受到分子和细胞水平上发生的各种复杂过程的影响。此外，麦角甾醇的生物合成过程对于实现对各种抑制剂的耐受性是重要的。我们的发现为分子工程以及筛选可能在生物乙醇发酵中有用的更健壮的酵母菌株提供了生物技术基础。
Lignocellulosic materials are abundant and among the most important potential sources for bioethanol production. Although the pretreatment of lignocellulose is necessary for efficient saccharification and fermentation, numerous by-products, including furan derivatives, weak acids, and phenolic compounds, are generated in the pretreatment step. Many of these components inhibit the growth and fermentation of yeast. In particular, vanillin is one of the most effective inhibitors in lignocellulose hydrolysates because it inhibits fermentation at very low concentrations. To identify the genes required for tolerance to vanillin, we screened a set of diploid yeast deletion mutants, which are powerful tools for clarifying the function of particular genes. Seventy-six deletion mutants were identified as vanillin-sensitive mutants. The numerous deleted genes in the vanillin-sensitive mutants were classified under the functional categories for 'chromatin remodeling' and 'vesicle transport', suggesting that these functions are important for vanillin tolerance. The cross-sensitivity of the vanillin-sensitive mutants to furan derivatives, weak acids, and phenolic compounds was also examined. Genes for ergosterol biosynthesis were required for tolerance to all inhibitory compounds tested, suggesting that ergosterol is a key component of tolerance to various inhibitors. Our analysis predicts that vanillin tolerance in Saccharomyces cerevisiae is affected by various complicated processes that take place on both the molecular and the cellular level. In addition, the ergosterol biosynthetic process is important for achieving a tolerance to various inhibitors. Our findings provide a biotechnological basis for the molecular engineering as well as for screening of more robust yeast strains that may potentially be useful in bioethanol fermentation.
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DOI：
10.1007/s10295-003-0049-x
发表时间：
2003-05-01
影响因子：
3.4
作者：
Saha, BC
通讯作者：
Saha, BC
影响因子：
30.8
作者：
Bennett, CB;Lewis, LK;Resnick, MA
通讯作者：
Resnick, MA
影响因子：
14.9
作者：
Fernandez-Ricaud L;Warringer J;Ericson E;Pylvänäinen I;Kemp GJ;Nerman O;Blomberg A
通讯作者：
Blomberg A
影响因子：
14.9
作者：
Durant, S;Karran, P
通讯作者：
Karran, P
影响因子：
5.4
作者：
Fitzgerald, DJ;Stratford, M;Narbad, A
通讯作者：
Narbad, A