MHVR-independent cell-cell spread of mouse hepatitis virus infection requires neutral pH fusion.
MHVR-independent cell-cell spread of mouse hepatitis virus infection requires neutral pH fusion.
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小鼠肝炎病毒感染的 MHVR 独立细胞传播需要中性 pH 融合。
DOI：
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发表时间：
1995
影响因子：
--
通讯作者：
M. Buchmeier
中科院分区：
文献类型：
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作者：
T. C. Nash;T. Gallagher;M. Buchmeier
受体特异性是病毒趋向性的关键决定因素。然而，在本报告中，我们已经证明MHV的细胞相关传播可以绕过与初级受体结合的要求，从而传播到对MHV感染具有抵抗力的细胞。抗受体抗体CC1可以阻断MHV病毒粒子的感染，但不能阻止MHV在受体阴性的BHK细胞或受体阳性的DBT细胞上的细胞相关传播。细胞相关MHV可能利用另一种低亲和力受体，这种受体不足以与MHV病毒粒子进行功能性相互作用。从理论上讲，MHV感染在体内通过受体独立、细胞相关的机制传播，为更广泛的宿主和组织范围提供了可能，尽管存在中和抗体，但感染仍有传播的可能。受体无关的、细胞相关的MHV传播需要中性pH融合能力。低ph依赖性MHV变体OBLV60利用内吞途径进入，不通过不依赖受体的机制传播。此外，阻断MHV尖峰介导的融合的抗病毒抗体抑制了细胞相关感染的传播。
Receptor-specificity is a key determinant of viral tropism. In this report, however, we have demonstrated that cell-associated spread of MHV can bypass the requirement for binding to primary receptors and thereby spread to cells that are resistant to MHV infection. Anti-receptor antibody CC1, which blocks infection by MHV virions, failed to prevent cell-associated spread of MHV to receptor-negative BHK cells or receptor-positive DBT cells. Cell-associated MHV may be utilizing an alternative, low-affinity receptor that is inadequate for functional interaction with MHV virions. Theoretically, dissemination of MHV infection through a receptor-independent, cell-associated mechanism in vivo provides the potential for broader host and tissue range, and for spread of infection despite the presence neutralizing antibodies. Receptor-independent, cell-associated spread of MHV requires neutral pH fusion capability. The low pH-dependent MHV variant OBLV60, which utilizes an endocytic route of entry, does not spread through a receptor-independent mechanism. Additionally, antiviral antibodies that block MHV spike-mediated fusion inhibited cell-associated spread of infection.
DOI：
10.1073/pnas.88.13.5533
发表时间：
1991-07-01
影响因子：
11.1
作者：
WILLIAMS, RK;JIANG, GS;HOLMES, KV
通讯作者：
HOLMES, KV