Enhanced pyruvic acid yield in an osmotic stress-resistant mutant of Yarrowia lipolytica
Enhanced pyruvic acid yield in an osmotic stress-resistant mutant of Yarrowia lipolytica
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解脂耶氏酵母抗渗透胁迫突变体中丙酮酸产量提高
DOI：
10.1016/j.ejbt.2020.01.002
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发表时间：
2020-01
影响因子：
2.7
通讯作者：
Sun Jie
中科院分区：
文献类型：
--
作者：
Yuan Wei;Lin Xiaoqiong;Zhong Shuang;Chen Junren;Wang Zhao;Sun Jie
背景：丙酮酸(PA)是一种重要的α-氧羧酸，在能量和碳代谢中起着重要作用。溶脂亚罗维菌(Yarrowia liolytica，Yarrowia liolytica)产油酵母具有较大的生产PA潜力。据报道，随着氯化钠浓度的增加，解脂耶尔森菌的生物量和PA产量都会增加。结果：采用常温和常温等离子体诱变方法，获得了耐盐溶脂假单胞菌A4突变株，提高了PA的产量。突变株A4在含有160g/L氯化钠的培养基上生长良好。在以甘油为唯一碳源的20-L发酵罐中，A4突变株发酵120h，PA产量达到97.2g/L(0.795 g/g甘油)，比出发菌株提高28.9%。结论：提高解脂耶尔森菌的耐盐性可以提高其PA产量。(C)2020年瓦尔帕莱索加托利卡大学。爱思唯尔B.V.制作和主办。保留所有权利。
Background: Pyruvic acid (PA), a vital alpha-oxocarboxylic acid, plays an important role in energy and carbon metabolism. The oleaginous yeast Yarrowia lipolytica (Y. lipolytica) has considerable potential for the production of PA. An increased NaCl concentration reportedly increases the biomass and PA yield of Y. lipolytica. Results: To increase the yield of PA, the NaCl-tolerant Y. lipolytica A4 mutant was produced using the atmospheric and room temperature plasma method of mutation. The A4 mutant showed growth on medium containing 160 g/L NaCl. The PA yield of the A4 mutant reached 97.2 g/L at 120 h (0.795 g/g glycerol) in a 20-L fermenter with glycerol as the sole carbon source, which was 28.9% higher than that of the parental strain. Conclusion: The PA yield from Y. lipolytica can be improved by increasing its NaCl tolerance. (C) 2020 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved.
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