Global proteomic analysis of two tick-borne emerging zoonotic agents: anaplasma phagocytophilum and ehrlichia chaffeensis.
Global proteomic analysis of two tick-borne emerging zoonotic agents: anaplasma phagocytophilum and ehrlichia chaffeensis.
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DOI：
10.3389/fmicb.2011.00024
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发表时间：
2011
影响因子：
5.2
通讯作者：
Rikihisa Y
中科院分区：
文献类型：
--
作者：
Lin M;Kikuchi T;Brewer HM;Norbeck AD;Rikihisa Y
嗜吞噬细胞无形体和查菲埃里希体是专性细胞内α-变形菌，感染人类白细胞，并引起潜在致命的新发人畜共患病。在本研究中，我们确定了这些细菌在人早幼粒细胞白血病细胞系HL-60中培养的全球蛋白质表达谱。质谱（MS）分析共鉴定出1，212个A。嗜吞噬细胞E. chaffeensis蛋白，分别占预测的细菌蛋白质组的89.3%和92.3%。几乎所有具有已知功能的细菌蛋白（≥99%）都表达，而在感染的人类细胞中仅检测到约80%的“假设”蛋白。定量MS/MS分析表明，在两种细菌中高表达的蛋白质包括分子伴侣，参与生物合成和代谢的酶，以及外膜蛋白，如A. P44和E. Chaffeensis P28/OMP-1。在113 A. p44旁系同源基因中有110个表达，88个为假基因编码。此外，HL-60细胞的细菌感染上调主要参与细胞骨架成分、囊泡运输、细胞信号传导和能量代谢的人类蛋白质的表达，但下调参与先天免疫的一些模式识别受体。我们的蛋白质组学数据代表了对A.嗜吞噬细胞菌和E. chaffeensis蛋白质组，并提供了由细菌感染调节的人类宿主蛋白质表达谱的定量视图。这些蛋白质组学数据的可用性将提供新的见解，这些专性细胞内病原体的生物学和发病机制。
Anaplasma phagocytophilum and Ehrlichia chaffeensis are obligatory intracellular α-proteobacteria that infect human leukocytes and cause potentially fatal emerging zoonoses. In the present study, we determined global protein expression profiles of these bacteria cultured in the human promyelocytic leukemia cell line, HL-60. Mass spectrometric (MS) analyses identified a total of 1,212 A. phagocytophilum and 1,021 E. chaffeensis proteins, representing 89.3 and 92.3% of the predicted bacterial proteomes, respectively. Nearly all bacterial proteins (≥99%) with known functions were expressed, whereas only approximately 80% of “hypothetical” proteins were detected in infected human cells. Quantitative MS/MS analyses indicated that highly expressed proteins in both bacteria included chaperones, enzymes involved in biosynthesis and metabolism, and outer membrane proteins, such as A. phagocytophilum P44 and E. chaffeensis P28/OMP-1. Among 113 A. phagocytophilum p44 paralogous genes, 110 of them were expressed and 88 of them were encoded by pseudogenes. In addition, bacterial infection of HL-60 cells up-regulated the expression of human proteins involved mostly in cytoskeleton components, vesicular trafficking, cell signaling, and energy metabolism, but down-regulated some pattern recognition receptors involved in innate immunity. Our proteomics data represent a comprehensive analysis of A. phagocytophilum and E. chaffeensis proteomes, and provide a quantitative view of human host protein expression profiles regulated by bacterial infection. The availability of these proteomic data will provide new insights into biology and pathogenesis of these obligatory intracellular pathogens.
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影响因子：
4.5
作者：
Dunning Hotopp JC;Lin M;Madupu R;Crabtree J;Angiuoli SV;Eisen JA;Seshadri R;Ren Q;Wu M;Utterback TR;Smith S;Lewis M;Khouri H;Zhang C;Niu H;Lin Q;Ohashi N;Zhi N;Nelson W;Brinkac LM;Dodson RJ;Rosovitz MJ;Sundaram J;Daugherty SC;Davidsen T;Durkin AS;Gwinn M;Haft DH;Selengut JD;Sullivan SA;Zafar N;Zhou L;Benahmed F;Forberger H;Halpin R;Mulligan S;Robinson J;White O;Rikihisa Y;Tettelin H
通讯作者：
Tettelin H
影响因子：
3.4
作者：
Cheng, Zhihui;Kumagai, Yumi;Rikihisa, Yasuko
通讯作者：
Rikihisa, Yasuko
影响因子：
3.2
作者：
Bao, Weichao;Kumagai, Yumi;Rikihisa, Yasuko
通讯作者：
Rikihisa, Yasuko
影响因子：
4.5
作者：
Blanc, Guillaume;Ogata, Hiroyuki;Robert, Catherine;Audic, Stephane;Suhre, Karsten;Vestris, Guy;Claverie, Jean-Michel;Raoult, Didier
通讯作者：
Raoult, Didier
影响因子：
14.9
作者：
Finn RD;Mistry J;Tate J;Coggill P;Heger A;Pollington JE;Gavin OL;Gunasekaran P;Ceric G;Forslund K;Holm L;Sonnhammer EL;Eddy SR;Bateman A
通讯作者：
Bateman A