Donor satellite cell engraftment is significantly augmented when the host niche is preserved and endogenous satellite cells are incapacitated.
Donor satellite cell engraftment is significantly augmented when the host niche is preserved and endogenous satellite cells are incapacitated.
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DOI：
10.1002/stem.1158
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发表时间：
2012-09
期刊：
影响因子：
5.2
通讯作者：
Morgan, Jennifer E.
中科院分区：
文献类型：
--
作者：
Boldrin, Luisa;Neal, Alice;Zammit, Peter S.;Muntoni, Francesco;Morgan, Jennifer E.
干细胞移植已经在某些遗传性疾病的临床实践中，是一种很有前途的治疗营养不良肌肉的方法。我们采用mdx小鼠杜氏肌营养不良模型，研究宿主卫星细胞生态位对供体肌肉干细胞（卫星细胞）对肌肉再生贡献的影响。我们发现，宿主卫星细胞的失能和肌肉生态位的保存促进了供体卫星细胞对肌肉再生和卫星细胞室功能重建的贡献。但是，如果寄主的生态位没有被及时补充，或者被有能力的寄主卫星细胞填满，它就会失去功能，供体移植就可以忽略不计了。将此方案应用于老龄宿主肌肉也能促进老龄供体卫星细胞的有效再生。相反，如果生态位被破坏，宿主卫星细胞仍然具有增殖能力，供体来源的移植就微不足道了。因此，在营养不良的人体肌肉中，保留卫星细胞的生态位，并伴随大多数卫星细胞的功能损伤，可能会提高干细胞治疗的效率。茎Cells2012;30:1971 - 1984
Stem cell transplantation is already in clinical practice for certain genetic diseases and is a promising therapy for dystrophic muscle. We used the mdx mouse model of Duchenne muscular dystrophy to investigate the effect of the host satellite cell niche on the contribution of donor muscle stem cells (satellite cells) to muscle regeneration. We found that incapacitation of the host satellite cells and preservation of the muscle niche promote donor satellite cell contribution to muscle regeneration and functional reconstitution of the satellite cell compartment. But, if the host niche is not promptly refilled, or is filled by competent host satellite cells, it becomes nonfunctional and donor engraftment is negligible. Application of this regimen to aged host muscles also promotes efficient regeneration from aged donor satellite cells. In contrast, if the niche is destroyed, yet host satellite cells remain proliferation-competent, donor-derived engraftment is trivial. Thus preservation of the satellite cell niche, concomitant with functional impairment of the majority of satellite cells within dystrophic human muscles, may improve the efficiency of stem cell therapy. Stem Cells2012;30:1971–1984
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影响因子：
5.2
作者：
Boldrin, Luisa;Zammit, Peter Steven;Morgan, Jennifer Elizabeth
通讯作者：
Morgan, Jennifer Elizabeth
DOI：
10.1016/j.mrrev.2010.01.007
发表时间：
2010-04-01
影响因子：
5.3
作者：
Harfouche, Ghida;Martin, Michele T.
通讯作者：
Martin, Michele T.
影响因子：
5.2
作者：
Collins, Charlotte A.;Zammit, Peter S.;Partridge, Terence A.
通讯作者：
Partridge, Terence A.
影响因子：
4.6
作者：
Hashimoto, N;Murase, T;Inagawa-Ogashiwa, M
通讯作者：
Inagawa-Ogashiwa, M
DOI：
10.1126/science.1191035
发表时间：
2010-08-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gilbert PM;Havenstrite KL;Magnusson KE;Sacco A;Leonardi NA;Kraft P;Nguyen NK;Thrun S;Lutolf MP;Blau HM
通讯作者：
Blau HM