Studies of alcohol-induced interdigitated gel phase in phosphatidylcholine multilamellar vesicles by the excimer method.
Studies of alcohol-induced interdigitated gel phase in phosphatidylcholine multilamellar vesicles by the excimer method.
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准分子法研究磷脂酰胆碱多层囊泡中酒精诱导的叉指凝胶相。
DOI：
10.1016/0005-2736(92)90226-c
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
T. Asano
中科院分区：
文献类型：
--
作者：
M. Yamazaki;M. Miyazu;T. Asano
我们开发了一种新的光谱方法（准分子法）来检测磷脂囊泡中的叉指凝胶相（L β1）。该方法基于芘磷脂酰胆碱（芘-PC）在L β1 相中准分子荧光峰的消失。使用这种方法，我们研究了在乙醇或乙二醇（EG）存在下二棕榈酰磷脂酰胆碱多层囊泡从凝胶相（L β'）到L β1 相的相变。在乙醇和EG两种情况下，通过X射线衍射法或扫描密度法测定，在相同浓度的从L β'到L β1的转变中，芘PC的准分子与单体荧光强度（EM）的比率急剧下降。过渡到 L β1 相后，EM 值变得非常低。这种准分子方法使我们能够检测到磷脂单层囊泡中的L β1 相，这是其他方法（例如X射线衍射）难以研究的。
We have developed a new spectroscopic method (excimer method) for detecting the interdigitated gel phase (L β1) in phospholipid vesicles. This method is based on the disappearance of an excimer fluorescence peak of pyrenephosphatidylcholine (pyrene-PC) in the L β1 phase. Using this method we have studied the phase transition from gel phase (L β′) to L β1 phase of dipalmitoylphosphatidylcholine multilamellar vesicles in the presence of ethanol or ethylene glycol (EG). In both the cases of ethanol and EG, a sharp decrease in the ratio of excimer to monomer fluorescence intensity (E M) of pyrene PC appeared at the same concentration of the transition from L β′ to L β1 as determined by the X-ray diffraction method or the scanning density method. After the transition to the L β1 phase, E M values became very low. This excimer method enables us to detect the L β1 phase in unilamellar vesicles of phospholipids, which can hardly be studied by other methods such as X-ray diffraction.
使用荧光团 1,6-二苯基-1,3,5-己三烯研究乙醇诱导的磷脂酰胆碱交错凝胶相。
影响因子：
2.9
作者：
Nambi,P;Rowe,ES;McIntosh,TJ
通讯作者：
McIntosh,TJ
叉指式烃链堆积导致脂质/醇悬浮液中的双相转变行为。
DOI：
10.1016/0005-2736(84)90562-5
发表时间：
1984
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Simon,SA;McIntosh,TJ
通讯作者：
McIntosh,TJ