Clinical significance of circulating immune cells in left- and right-sided colon cancer.
Clinical significance of circulating immune cells in left- and right-sided colon cancer.
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循环免疫细胞在左右结肠癌中的临床意义
作者：
Di J;Zhuang M;Yang H;Jiang B;Wang Z;Su X
左侧和右侧结肠癌（分别为lcc和rcc）在其流行病学，发病机制，遗传和表观遗传改变，分子途径和预后方面存在差异。值得注意的是，免疫反应基因表达谱在小细胞癌患者和小细胞癌患者之间存在差异。免疫系统在肿瘤免疫监测中起着重要作用，越来越多的证据表明外周血免疫细胞对肿瘤预后有深远的影响。本研究旨在确定循环免疫细胞与结肠肿瘤位置的临床意义。方法采用流式细胞术对不同类型的循环免疫细胞进行分离和表面标记分析。我们比较了94例RCC或LCC患者外周血中树突状细胞（dc）和T细胞亚群的数量，并分析了这些免疫细胞的比例与肿瘤分期、肿瘤分化和淋巴转移的关系。结果在肿瘤晚期，LCC患者的循环髓样DCs （P = 0.049）和浆细胞样DCs （P = 0.018）水平高于RCC患者。在低分化肿瘤中，LCC患者浆细胞样dc （P = 0.036）、CD4+记忆T （Tm）细胞（P = 0.012）、CD4+ T细胞（P = 0.028）、Tm细胞（P = 0.014）和调节性T细胞（P = 0.001）的数量明显高于RCC患者。在LCC或RCC患者的晚期，循环CD4+ T细胞、Tm细胞和CD4+ Tm细胞水平显著升高，而在RCC患者的低分化肿瘤中，这些细胞水平下降。此外，CD4+ Tm细胞和CD4+ T细胞水平与LCC和RCC患者的淋巴结转移有显著相关性。循环免疫细胞与肿瘤部位、分期、分化相关，可用于预测结肠癌患者淋巴转移。这种全身免疫的变化可能导致结肠癌患者预后的差异。
Background Left-sided and right-sided colon cancers (LCCs and RCCs, respectively) differ in their epidemiology, pathogenesis, genetic and epigenetic alterations, molecular pathways and prognosis. Notably, immune response gene expression profiles have been shown to differ between patients with LCC and patients with RCC. The immune system plays an important role in tumor immunosurveillance, and there is increasing evidence that peripheral blood immune cells have a profound influence on tumor prognosis. This study aimed to determine the clinical significance of circulating immune cells with respect to colon tumor locations. Methods Different types of circulating immune cells were separated and analysed based on their surface markers by flow cytometry. We compared the numbers of dendritic cells (DCs) and T cell subsets in the peripheral blood of 94 patients with RCC or LCC and analysed the proportions of these immune cells in relation to tumor stage, tumor differentiation and lymphatic metastasis. Results We show that at later tumor stages, patients with LCC had higher levels of circulating myeloid DCs (P = 0.049) and plasmacytoid DCs (P = 0.018) than patients with RCC. In poorly differentiated tumors, LCC patients had significantly higher amount of plasmacytoid DCs (P = 0.036), CD4+ memory T (Tm) cells (P = 0.012), CD4+ T cells (P = 0.028), Tm cells (P = 0.014), and regulatory T cells (P = 0.001) than RCC patients. The levels of circulating CD4+ T cells, Tm cells and CD4+ Tm cells were significantly elevated at later stages in patients with LCC or RCC, while these cells decreased in poorly differentiated tumors in patients with RCC. Moreover, CD4+ Tm cell and CD4+ T cell levels are significantly associated with lymph node metastasis in patients with LCC and RCC. Discussion Circulating immune cells were associated with tumor location, tumor stage and tumor differentiation, and can be used to predict lymphatic metastasis in patients with colon cancer. This variation in systemic immunity could contribute to the differential prognosis of patients with colon cancer.
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影响因子：
64.8
作者：
Headley MB;Bins A;Nip A;Roberts EW;Looney MR;Gerard A;Krummel MF
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Krummel MF
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Iacopetta, Barry