The Role of Pyoluteorin from Pseudomonas protegens Pf-5 in Suppressing the Growth and Pathogenicity of Pantoea ananatis on Maize
The Role of Pyoluteorin from Pseudomonas protegens Pf-5 in Suppressing the Growth and Pathogenicity of Pantoea ananatis on Maize
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DOI：
10.3390/ijms23126431
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发表时间：
2022-06-09
影响因子：
5.6
通讯作者：
Wu, Huijun
中科院分区：
文献类型：
--
作者：
Gu, Qin;Qiao, Junqing;Wang, Ruoyi;Lu, Juan;Wang, Zhengqi;Li, Pingping;Zhang, Lulu;Ali, Qurban;Khan, Abdur Rashid;Gao, Xuewen;Wu, Huijun
根际细菌Protegens Pf-5能定殖于植物的种子和根表面，并能保护植物免受病原菌的侵染。Pf-5产生的次级代谢产物包括脂肽和聚酮，参与其生防活性。我们从Pf-5中分离出粗提取物。该化合物对玉米褐腐病病原菌Pantoea ananatis DZ-12具有较强的抑菌活性。HPLC分析和活性测定结果表明，粗提物中的聚酮吡咯烷酮参与了DZ-12的生长抑制作用，脂肽orfamide A是粗提物中的主要生物表面活性剂。进一步的研究表明，粗提物中的热解酚能显著抑制DZ-12细胞生物膜的形成，并诱导DZ-12细胞内活性氧的积累。扫描电镜和透射电镜观察表明，粗提物对病原菌细胞有严重的破坏作用，并引起细胞质外渗和空洞化。Pf-5粗提物能显著降低DZ-12对玉米叶片的致病性，且呈剂量依赖关系。聚酮绿脓菌素对DZ-12具有较强的抗菌活性，具有开发为抗菌剂的潜力。
The rhizospheric bacterium Pseudomonas protegens Pf-5 can colonize the seed and root surfaces of plants, and can protect them from pathogen infection. Secondary metabolites, including lipopeptides and polyketides produced by Pf-5, are involved in its biocontrol activity. We isolated a crude extract from Pf-5. It exhibited significant surface activity and strong antibacterial activity against Pantoea ananatis DZ-12, which causes maize brown rot on leaves. HPLC analysis combined with activity tests showed that the polyketide pyoluteorin in the crude extract participated in the suppression of DZ-12 growth, and that the lipopeptide orfamide A was the major biosurfactant in the crude extract. Further studies indicated that the pyoluteorin in the crude extract significantly suppressed the biofilm formation of DZ-12, and it induced the accumulation of reactive oxygen species in DZ-12 cells. Scanning electron microscopy and transmission electron microscopy observation revealed that the crude extract severely damaged the pathogen cells and caused cytoplasmic extravasations and hollowing of the cells. The pathogenicity of DZ-12 on maize leaves was significantly reduced by the crude extract from Pf-5 in a dose-dependent manner. The polyketide pyoluteorin had strong antibacterial activity against DZ-12, and it has the potential for development as an antimicrobial agent.
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