Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5.
Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5.
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DOI：
10.1038/nbt1110
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发表时间：
2005-07
影响因子：
46.9
通讯作者：
Loper JE
中科院分区：
文献类型：
--
作者：
Paulsen IT;Press CM;Ravel J;Kobayashi DY;Myers GS;Mavrodi DV;DeBoy RT;Seshadri R;Ren Q;Madupu R;Dodson RJ;Durkin AS;Brinkac LM;Daugherty SC;Sullivan SA;Rosovitz MJ;Gwinn ML;Zhou L;Schneider DJ;Cartinhour SW;Nelson WC;Weidman J;Watkins K;Tran K;Khouri H;Pierson EA;Pierson LS 3rd;Thomashow LS;Loper JE
荧光假单胞菌Pf-5是一种植物共生细菌，栖息在根际，产生次生代谢物，抑制土壤传播的植物病原体。确定了7.1 mb的Pf-5全基因组序列。我们分析了重复序列以确定基因组岛，与其他方法一起，表明荧光p.f n5最近的横向获取包括6个次级代谢物基因簇，7个噬菌体区域和一个移动基因组岛。我们确定了其在植物上的共生生活方式的各种特征，包括利用植物衍生化合物的广泛分解代谢和运输能力，使用多种铁铁载体的明显能力，解毒系统以保护免受氧化应激，以及缺乏III型分泌系统和相关病原体中发现的毒素。除了已知的荧光假单胞菌Pf-5产生的6种次生代谢物外，还鉴定了3种新的次生代谢物生物合成基因簇，这些基因簇可能与荧光假单胞菌Pf-5的生物防治特性有关。本文的在线版本（doi:10.1038/nbt1110）包含补充材料，可供授权用户使用。
Pseudomonas fluorescens Pf-5 is a plant commensal bacterium that inhabits the rhizosphere and produces secondary metabolites that suppress soilborne plant pathogens. The complete sequence of the 7.1-Mb Pf-5 genome was determined. We analyzed repeat sequences to identify genomic islands that, together with other approaches, suggested P. fluorescens Pf-5's recent lateral acquisitions include six secondary metabolite gene clusters, seven phage regions and a mobile genomic island. We identified various features that contribute to its commensal lifestyle on plants, including broad catabolic and transport capabilities for utilizing plant-derived compounds, the apparent ability to use a diversity of iron siderophores, detoxification systems to protect from oxidative stress, and the lack of a type III secretion system and toxins found in related pathogens. In addition to six known secondary metabolites produced by P. fluorescens Pf-5, three novel secondary metabolite biosynthesis gene clusters were also identified that may contribute to the biocontrol properties of P. fluorescens Pf-5. The online version of this article (doi:10.1038/nbt1110) contains supplementary material, which is available to authorized users.
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影响因子：
4.4
作者：
de Souza, JT;de Boer, M;Raaijmakers, JM
通讯作者：
Raaijmakers, JM
DOI：
10.1073/pnas.1731982100
发表时间：
2003-09-02
影响因子：
11.1
作者：
Buell, CR;Joardar, V;Collmer, A
通讯作者：
Collmer, A
影响因子：
3.2
作者：
CASTRIC, PA
通讯作者：
CASTRIC, PA
影响因子：
3.2
作者：
Laville, J;Blumer, C;Haas, D
通讯作者：
Haas, D
影响因子：
3.2
作者：
KRAUS, J;LOPER, JE
通讯作者：
LOPER, JE