Phylogenetic Position of Haemaphysalis kashmirensis and Haemaphysalis cornupunctata, with Notes on Rickettsia spp.
Phylogenetic Position of Haemaphysalis kashmirensis and Haemaphysalis cornupunctata, with Notes on Rickettsia spp.
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尽管在东方地区有很高的多样性，但血蜱属蜱的遗传数据和媒介潜力一直被忽视。本研究的目的是遗传特征的3种血蜱属：血蜱cornupunctata，血蜱kashmirensis，血蜱montgomeryi感染山羊和绵羊，立克次体。在巴基斯坦兴都库什山脉喜马拉雅山脉与这些蜱类物种有关。共检获蜱834只，其中山羊64只（53.3%），绵羊56只（46.6%），有蜱86只（71.6%）。对形态学鉴定的蜱进行DNA提取和PCR扩增，获得16 SrDNA和考克斯片段。立克次体属通过扩增gltA、ompA和ompB部分片段检测与所收集蜱相关的病毒。16 S rDNA序列分析表明，H. cornupunctata和H. montgomeryi的16 SrDNA序列与同一物种的16 SrDNA序列同源性最高达100%; kashmirensis与沟血蜱的同源性最高，为93-95%。H.的考克斯序列。Montgomeryi与同一物种具有100%的同一性。与之相比，H. cornupunctata和H. kashmirensis与Haemaphysalis punctata的同源性最高为87.65-89.22%，与H. sulcata，分别。对来自H.喀什米尔立克次体与康氏立克次体亚种的同源性最高，达97.89%。结果表明，ompA和ompB片段与Rickettsia sp.和“长角立克次体”的同源性分别为100%和98.16%。另一个gltA序列从H.结果表明，孟氏立克次体与霍氏立克次体的ompA和ompB序列具有100%的同源性; Hoogstraalii没有成功。在系统发育树中，H. cornupunctata与相应种聚在一起，而考克斯与H.斑点。16 S rDNA和考克斯序列分析表明，H. kashmirensis与H. sulcata。立克次体gltA序列在斑点热群中单独聚为一类，而立克次体gltA序列在斑点热群中聚为一类。Hoogstraalii与立克次体过渡群中的同种聚在一起。在SF组中，立克次体ompA和ompB序列分别与未确定的立克次体和“长角立克次体”聚簇。这是关于H.喀什米尔人。本研究表明，血蜱属蜱类具有窝藏和/或传播立克次体的潜力。本地区
Despite high diversity in the Oriental region, ticks of the genus Haemaphysalis have been neglected regarding their genetic data and vector potential. This study aimed to genetically characterize three species of the genus Haemaphysalis: Haemaphysalis cornupunctata, Haemaphysalis kashmirensis, and Haemaphysalis montgomeryi infesting goats and sheep, and Rickettsia spp. associated with these tick species in the Hindu Kush Himalayan range of Pakistan. Altogether, 834 ticks were collected by examining 120 hosts including goats (64/120, 53.3%) and sheep (56/120, 46.6%), in which 86 (71.6%) hosts were found to be tick-infested. The morphologically identified ticks were subjected to DNA extraction and PCR for the amplification of partial 16S rDNA and cox fragments. Rickettsia spp. associated with the collected ticks were detected through the amplification of gltA, ompA and ompB partial fragments. The 16S rDNA of H. cornupunctata and H. montgomeryi showed a maximum identity of 100% with the sequences of the same species, whereas the 16S rDNA of H. kashmirensis showed the highest identity of 93–95% with Haemaphysalis sulcata. The cox sequence of H. montgomeryi displayed 100% identity with the same species. In comparison, the cox sequences of H. cornupunctata and H. kashmirensis showed maximum identities of 87.65–89.22% with Haemaphysalis punctata and 89.34% with H. sulcata, respectively. The gltA sequence of Rickettsia sp. from H. kashmirensis showed the highest identity of 97.89% with Rickettsia conorii subsp. raoultii, while the ompA and ompB fragments from the same DNA samples revealed 100% and 98.16% identity with Rickettsia sp. and “Candidatus Rickettsia longicornii”, respectively. Another gltA sequence amplified from H. montgomeryi ticks showed 100% identity with Rickettsia hoogstraalii, while the attempts to amplify ompA and ompB for R. hoogstraalii were unsuccessful. In the phylogenetic tree, the 16S rDNA of H. cornupunctata clustered with the corresponding species while its cox clustered with H. punctata. Both 16S rDNA and cox sequences of H. kashmirensis clustered with H. sulcata. The gltA sequence of Rickettsia sp. was clustered individually in the spotted fever (SF) group of Rickettsia, while the gltA sequence of R. hoogstraalii was clustered with the same species in the transition group of Rickettsia. In the SF group, the rickettsial ompA and ompB sequence clustered with undetermined Rickettsia sp. and “Candidatus Rickettsia longicornii”, respectively. This is the earliest study regarding the genetic characterization of H. kashmirensis. This study indicated that ticks belong to the genus Haemaphysalis have the potential of harboring and/or transmitting Rickettsia spp. in the region.
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影响因子：
1.3
作者：
HOOGSTRAAL, H;MCCARTHY, VC
通讯作者：
MCCARTHY, VC
影响因子：
3.2
作者：
Igolkina, Yana;Rar, Vera;Tikunova, Nina
通讯作者：
Tikunova, Nina
影响因子：
2.4
作者：
Jongejan, F;Uilenberg, G
通讯作者：
Uilenberg, G
影响因子：
1.9
作者：
Guan, Guiquan;Moreau, Emmanuelle;Yin, Hong
通讯作者：
Yin, Hong
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro