Diverse responses of Symbiodinium types to menthol and DCMU treatment.
Diverse responses of Symbiodinium types to menthol and DCMU treatment.
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作者：
Wang JT;Keshavmurthy S;Chu TY;Chen CA
为了了解共生藻类在胁迫下光合作用抑制和活性氧（ROS）产生的机制，广泛使用诸如二氯苯基二甲基脲（DCMU）的化学品。此外，DCMU和最近的薄荷醇被用来产生脱共生刺胞动物宿主。虽然DCMU对共生藻细胞的影响已被广泛研究，但没有研究表明薄荷醇诱导珊瑚漂白背后的机制。此外，没有研究比较DCMU和薄荷醇处理对光系统II（PSII）活性和不同共生藻类型中ROS产生的影响。在这项研究中，我们利用了五个新鲜分离的共生藻类型（S。minutum（B1）、S. goreaui（C1）、C3、C15和S. trenchii（D1a））以比较DCMU和薄荷醇处理的效果。将共生藻细胞暴露于不同浓度的DCMU和薄荷醇4h。结果显示，对于PSII抑制，薄荷醇处理的细胞的50%抑制浓度（IC 50）值为0.72 μ M至1.96 μ M，而DCMU处理的细胞的IC 50值为29 μ M至74 μ M。共生藻型的不同响应表现在对薄荷醇的PSII耐受性方面（微小共生藻>川氏共生藻= C15 > C3 =戈氏共生藻），并且还表现在响应曲线中。相反，当不同类型的患者用DCMU治疗时，反应并不如此不同。五个薄荷醇处理的Symbiodinium类型显示即时和显着的ROS生成时，PSII活性被抑制，相比没有ROS生成DCMU处理的Symbiodinium类型。这两个结果表明，薄荷醇抑制共生藻PSII活性通过共生藻类型依赖的机制，这也是不同于与DCMU处理。本研究进一步证实了光合功能共生藻即使在同一进化枝内也具有不同的逆境响应。
To understand the mechanism of photosynthetic inhibition and generation of reactive oxygen species (ROS) in Symbiodinium types under stress, chemicals such as dichlorophenyl dimethylurea (DCMU) are widely used. Moreover, DCMU and recently menthol were used to generate aposymbiotic cnidarian hosts. While the effects of DCMU on Symbiodinium cells have been extensively studied, no studies have shown the mechanism behind menthol-induced coral bleaching. Moreover, no study has compared the effects of DCMU and menthol treatments on photosystem II (PSII) activity and generation of ROS in different Symbiodinium types. In this study, we utilized five freshly isolated Symbiodinium types (S. minutum (B1), S. goreaui (C1), C3, C15, and S. trenchii (D1a)) to compare the effects of DCMU and menthol treatments. Symbiodinium cells were exposed to DCMU and menthol at different concentrations for 4 h. Results showed that values of the 50% inhibitory concentration (IC50) for PSII inhibition were 0.72∼1.96 mM for menthol-treated cells compared to 29∼74 pM for DCMU-treated cells. Diverse responses of Symbiodinium types were displayed in terms of PSII tolerance to menthol (S. minutum > S. trenchii = C15 > C3 = S. goreaui), and also in the response curves. In contrast, responses were not so diverse when the different types were treated with DCMU. Three of five menthol-treated Symbiodinium types showed instant and significant ROS generation when PSII activity was inhibited, compared to no ROS being generated in DCMU-treated Symbiodinium types. Both results indicated that menthol inhibited Symbiodinium PSII activity through Symbiodinium type-dependent mechanisms, which were also distinct from those with DCMU treatment. This study further confirmed that photosynthetic functions Symbiodinium have diverse responses to stress even within the same clade.
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防晒霜通过促进病毒感染引起珊瑚漂白。
影响因子：
10.4
作者：
Danovaro, Roberto;Bongiorni, Lucia;Corinaldesi, Cinzia;Giovannelli, Donato;Damiani, Elisabetta;Astolfi, Paola;Greci, Lucedio;Pusceddu, Antonio
通讯作者：
Pusceddu, Antonio
影响因子：
2.5
作者：
Jones, RJ
通讯作者：
Jones, RJ
DOI：
10.1017/s0025315400032173
发表时间：
1989-11-01
影响因子：
1.2
作者：
MOORE, SJ
通讯作者：
MOORE, SJ
影响因子：
7.3
作者：
Jones, RJ;Hoegh-Guldberg, O;Schreiber, U
通讯作者：
Schreiber, U
影响因子：
2.5
作者：
Dani, Vincent;Priouzeau, Fabrice;Sabourault, Cecile
通讯作者：
Sabourault, Cecile