Endothelial biocompatibility and accumulation of SPION under flow conditions
Endothelial biocompatibility and accumulation of SPION under flow conditions
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流动条件下内皮生物相容性和 SPION 的积累
DOI：
10.1016/j.jmmm.2014.09.005
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发表时间：
2015
影响因子：
2.7
通讯作者：
Cicha I
中科院分区：
文献类型：
--
作者：
Matuszak J;Zaloga J;Friedrich RP;Lyer S;Nowak J;Odenbach S;Alexiou C;Cicha I
磁性靶向被认为是在动脉粥样硬化病变部位积累纳米颗粒的一种很有前途的方法，但磁性纳米颗粒对血管壁的生物学效应知之甚少。在这里，我们使用两种互补的方法：实时细胞分析和活细胞显微镜，研究了SPION（0-60µg/mL）处理后内皮细胞的生长和活力。此外，在动脉分叉的蛋白体外模型中评估了循环超顺磁性氧化铁纳米颗粒（SPIONs）的摄取。在测试浓度下，SPIONs耐受性良好，对内皮细胞生长没有重大影响。我们的研究结果进一步表明，内皮细胞SPION摄取的均匀分布与通道几何形状或血流动力学条件无关：在没有磁力的情况下，在分叉外壁的非均匀剪切应力区域没有观察到SPION积累的增加。外部磁体的应用增强了SPIONs在非均匀剪切应力区域的积累。内皮细胞（ECs）能够很好地耐受非均匀剪切应力区SPIONs摄取的增加，并且不影响内皮细胞的活力或附着。这些发现表明，磁性靶向可以构成一种有前途和安全的技术，用于向动脉粥样硬化病变输送成像和治疗纳米颗粒。
Magnetic targeting is considered a promising method to accumulate the nanoparticles at the sites of atherosclerotic lesions, but little is known about the biological effects of magnetic nanoparticles on the vascular wall. Here, we investigated endothelial cell growth and vitality upon treatment with SPION (0–60 µg/mL) using two complementing methods: real-time cell analysis and live-cell microscopy. Moreover, the uptake of circulating superparamagnetic iron oxide nanoparticles (SPIONs) was assessed in anin vitromodel of arterial bifurcations.At the tested concentrations, SPIONs were well tolerated and had no major influence on endothelial cell growth. Our results further showed a uniform distribution of endothelial SPION uptake independent of channel geometry or hemodynamic conditions: In the absence of magnetic force, no increase in accumulation of SPIONs at non-uniform shear stress region at the outer walls of bifurcation was observed. Application of external magnet allowed enhanced accumulation of SPIONs at the regions of non-uniform shear stress. Increased uptake of SPIONs at non-uniform shear stress region was well tolerated by endothelial cells (ECs) and did not affect endothelial cell viability or attachment. These findings indicate that magnetic targeting can constitute a promising and safe technique for the delivery of imaging and therapeutic nanoparticles to atherosclerotic lesions.
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影响因子：
5.3
作者：
Cicha, Iwona;Beronov, Kamen;Garlichs, Christoph D.
通讯作者：
Garlichs, Christoph D.
影响因子：
5.6
作者：
Janko C;Dürr S;Munoz LE;Lyer S;Chaurio R;Tietze R;Löhneysen Sv;Schorn C;Herrmann M;Alexiou C
通讯作者：
Alexiou C
剪切应力模式在 TNF-α 诱导的致动脉粥样硬化蛋白表达和单核细胞与内皮粘附中的作用。
影响因子：
2.1
作者：
K. Urschel;Anja Wörner;W. Daniel;C. Garlichs;I. Cicha
通讯作者：
I. Cicha
影响因子：
2.7
作者：
Tietze, Rainer;Jurgons, Roland;Alexiou, Christoph
通讯作者：
Alexiou, Christoph