In Vivo Interaction Between Mitochondria Carrying mtDNAs From Different Mouse Species
In Vivo Interaction Between Mitochondria Carrying mtDNAs From Different Mouse Species
复制标题
携带不同小鼠 mtDNA 的线粒体之间的体内相互作用
作者：
A. Sato;K. Nakada;H. Shitara;H. Yonekawa;J. Hayashi
以携带小鼠线粒体DNA（mtDNA）的B6mtspr品系小鼠的受精卵为受体，分别接种携带野生型和缺失突变型线粒体DNA（ΔmtDNA）的小鼠，建立线粒体病模型小鼠mitomice。臀肌在这些实验中，来自不同小鼠物种的mtDNA用于鉴定线粒体中的外源和内源野生型mtDNA。结果表明，外源ΔmtDNA能传递M. M.通过雌性生殖细胞系传给下一代。外源性野生型mtDNA的完全消除可能是由于随机分离，而外源性ΔmtDNA的传递可能是由于其较小的尺寸导致繁殖优势。携带外源ΔmtDNA的F3代线粒体组织表现出对呼吸缺陷的保护作用，直到ΔmtDNA大量积累。这种对线粒体功能障碍表达的保护作用是在M. spretus，因为mitomice不具有外源野生型M. M.阿提库斯这些观察结果为携带ΔmtDNA的外源性线粒体和携带M的内源性线粒体之间存在相互作用提供了明确的证据。spretus野生型mtDNA。
Mitochondrial disease model mice, mitomice, were created using zygotes of B6mtspr strain mice carrying mitochondrial DNA (mtDNA) from Mus spretus as recipients of exogenous mitochondria carrying wild-type and a deletion mutant mtDNA (ΔmtDNA) of M. musculus domesticus. In these experiments, mtDNAs from different mouse species were used for identification of exo- and endogenous wild-type mtDNAs in the mitomice. Results showed transmission of exogenous ΔmtDNA, but not exogenous wild-type mtDNA, of M. m. domesticus to following generations through the female germ line. Complete elimination of exogenous wild-type mtDNA would be due to stochastic segregation, whereas transmission of exogenous ΔmtDNA would be due to its smaller size leading to a propagational advantage. Tissues in mitomice of the F3 generation carrying exogenous ΔmtDNA showed protection from respiration defects until ΔmtDNA accumulated predominantly. This protection from expression of mitochondrial dysfunction was attained with the help of endogenous wild-type mtDNA of M. spretus, since mitomice did not possess exogenous wild-type mtDNA of M. m. domesticus. These observations provide unambiguous evidence for the presence of interaction between exogenous mitochondria carrying ΔmtDNA and endogenous mitochondria carrying M. spretus wild-type mtDNA.
影响因子：
56.9
作者：
WALLACE, DC
通讯作者：
WALLACE, DC