Functional Roles of NOD1 in Odontoblasts on Dental Pulp Innate Immunity.
Functional Roles of NOD1 in Odontoblasts on Dental Pulp Innate Immunity.
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DOI：
10.1155/2016/9325436
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发表时间：
2016
影响因子：
--
通讯作者：
Matsuo T
中科院分区：
文献类型：
--
作者：
Hosokawa Y;Hirao K;Yumoto H;Washio A;Nakanishi T;Takegawa D;Kitamura C;Matsuo T
与龋齿相关的病原体首先被成牙本质细胞识别并诱导炎症事件发展为牙髓炎。一般来说，微生物病原体的初始感知是由模式识别受体介导的，例如Toll样受体和核苷酸结合寡聚结构域（NOD）；然而，人们对成牙本质细胞中的 NOD 知之甚少。在本研究中，通过流式细胞术评估大鼠成牙本质细胞系 KN-3 中表达的 NOD 水平，并通过实时 PCR 和 ELISA 分析 NOD 特异性配体刺激的 KN-3 细胞中趋化因子的水平。通过特异性抑制剂的阻断测定和报告基因测定来评估NOD特异性配体激活的信号转导途径。在KN-3细胞中，NOD1的表达水平强于NOD2，并且趋化因子（例如CINC-1、CINC-2、CCL20和MCP-1）的产生通过NOD1特异性配体的刺激而上调，但NOD2特异性配体的刺激则不上调。 p38 MAPK 和 AP-1 信号抑制剂可减少 NOD1 特异性配体刺激产生的 CINC-2 和 CCL20。此外，报告基因检测证明 AP-1 在 NOD1 特异性配体刺激的 KN-3 细胞中激活。这些发现表明，成牙本质细胞中表达的NOD1通过p38-AP-1信号通路上调趋化因子的表达，提示NOD1可能在牙髓炎的发生和发展中发挥重要作用。
Caries-related pathogens are first recognized by odontoblasts and induce inflammatory events that develop to pulpitis. Generally, initial sensing of microbial pathogens is mediated by pattern recognition receptors, such as Toll-like receptor and nucleotide-binding oligomerization domain (NOD); however, little is known about NODs in odontoblasts. In this study, the levels of NODs expressed in rat odontoblastic cell line, KN-3, were assessed by flow cytometry and the levels of chemokines in NOD-specific ligand-stimulated KN-3 cells were analyzed by real-time PCR and ELISA. The signal transduction pathway activated with NOD-specific ligand was assessed by blocking assay with specific inhibitors and reporter assay. In KN-3 cells, the expression level of NOD1 was stronger than that of NOD2 and the production of chemokines, such as CINC-1, CINC-2, CCL20, and MCP-1, was upregulated by stimulation with NOD1-specific ligand, but not with NOD2-specific ligand. CINC-2 and CCL20 production by stimulation with NOD1-specific ligand was reduced by p38 MAPK and AP-1 signaling inhibitors. Furthermore, the reporter assay demonstrated AP-1 activation in NOD1-specific ligand-stimulated KN-3 cells. These findings indicated that NOD1 expressed in odontoblasts functions to upregulate the chemokines expression via p38-AP-1 signaling pathway and suggested that NOD1 may play important roles in the initiation and progression of pulpitis.
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影响因子：
7.6
作者：
Hirao, K.;Yumoto, H.;Matsuo, T.
通讯作者：
Matsuo, T.
影响因子：
5
作者：
ElSalhy, M.;Azizieh, F.;Raghupathy, R.
通讯作者：
Raghupathy, R.
影响因子：
3.2
作者：
Keller, Jean-Francois;Carrouel, Florence;Farges, Jean-Christophe
通讯作者：
Farges, Jean-Christophe
影响因子：
7.6
作者：
Horst, O. V.;Tompkins, K. A.;Dale, B. A.
通讯作者：
Dale, B. A.
影响因子：
4.2
作者：
Nakanishi, T;Takahashi, K;Matsuo, T
通讯作者：
Matsuo, T