Artificial liver: A forthcoming attraction
Artificial liver: A forthcoming attraction
复制标题
人工肝：即将到来的吸引力
作者：
N. Sussman;James H. Kelly
我们研制了一种新型的中空纤维肝细胞生物反应器，作为一种潜在的生物人工肝。将新鲜收获的大鼠肝细胞包埋在灌注生物反应器中中空纤维内的三维凝胶基质中。凝胶包埋允许细胞以高密度培养，同时保持组织特异性功能。在10个生物反应器中评价肝细胞功能，每个生物反应器含有约5 x 107个细胞。耗氧量平均为0.32 pmole/cell/hr，白蛋白外观平均为0.60 pg/cell/hr，利多卡因清除率（P-450活性的测量）平均为0.74 pg/cell/hr。在第7天的电子显微镜下显示出独特的超微结构的活的，分化的肝细胞：胆小管，细胞间连接，过氧化物酶体，丰富的线粒体和糖原颗粒。组织特异性功能和超微结构的维持表明，这种生物反应器配置具有作为支持肝功能衰竭患者以及体外研究肝细胞的装置的潜力。
We have developed a novel hepatocyte loaded hollow fiber bioreactor as a potential bioartificial liver. Freshly harvested rat hepatocytes were entrapped in a three‐dimensional gel matrix within hollow fibers in a perfused bioreactor. Gel entrapment allowed cells to be cultured at high density while maintaining tissuespecific function. Hepatocyte function was evaluated in 10 bioreactors, each containing approximately 5 x 107 cells. Oxygen consumption averaged 0.32 pmole/cell/hr, albumin appearance averaged 0.60 pg/cell/hr, and lidocaine clearance (a measure of the P‐450 activity) averaged 0.74 pg/cell/hr. Function persisted for the 7 days of the study. Electron microscopy at 7 days showed the distinctive ultrastructure of viable, differentiated hepatocytes: bile canaliculi, intercellular junctions, peroxisomes, abundant mitochondria, and glycogen granules. Maintenance of tissue specific function and ultrastructure suggests that this bioreactor configuration has potential as a device to support patients in liver failure, as well as to study hepatocytes in vitro.
影响因子：
2.4
作者：
Kelly,JH;Koussayer,T;He,D;Chong,MG;Shang,TA;Whisennand,HH;Sussman,NL
通讯作者：
Sussman,NL