Metabolic recovery of Arabidopsis thaliana roots following cessation of oxidative stress.
Metabolic recovery of Arabidopsis thaliana roots following cessation of oxidative stress.
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DOI：
10.1007/s11306-011-0296-1
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发表时间：
2012-02
期刊：
影响因子：
3.6
通讯作者：
Obata, Toshihiro
中科院分区：
文献类型：
--
作者：
Lehmann, Martin;Laxa, Miriam;Sweetlove, Lee J.;Fernie, Alisdair R.;Obata, Toshihiro
为了科普各种环境胁迫导致的活性氧（ROS）的产生，植物代谢被称为是在不同的胁迫下，具体改变。在克服压力后，新陈代谢应该被重新配置以恢复基础运作，然而关于如何实现这一点的知识是粗略的。为了研究氧化胁迫后根系的代谢恢复，分析了代谢物丰度和碳流的变化。用甲萘醌处理拟南芥根以引起氧化胁迫。用~（13）C标记的葡萄糖灌根，测定碳同位素的再分配，研究碳流。氧化胁迫下，通过糖酵解、三羧酸循环和氨基酸代谢等途径的标记物再分配减少。去除甲萘醌后，许多与压力相关的变化恢复到基础水平。已糖磷酸盐、苹果酸盐、2-酮戊二酸盐、谷氨酸盐和天冬氨酸盐的量的减少完全恢复或甚至增加至高于对照水平。然而，一些代谢产物如戊糖磷酸盐和柠檬酸盐没有恢复，而是保持其水平甚至进一步增加。标记物再分布的改变在很大程度上与代谢物丰度相关。糖酵解碳流量恢复到控制水平，仅18小时后，甲萘醌去除，虽然TCA循环和一些氨基酸，如天冬氨酸和谷氨酸需要更长的时间才能恢复。两者合计，植物根系代谢被证明能够克服甲萘醌诱导的氧化应激与独立的途径所需的不同的时间段暗示参与途径特异性的调节过程。本文的在线版本（doi：10.1007/s11306-011-0296-1）包含补充材料，可供授权用户使用。
To cope with the various environmental stresses resulting in reactive oxygen species (ROS) production plant metabolism is known to be altered specifically under different stresses. After overcoming the stress the metabolism should be reconfigured to recover basal operation however knowledge concerning how this is achieved is cursory. To investigate the metabolic recovery of roots following oxidative stress, changes in metabolite abundance and carbon flow were analysed. Arabidopsis roots were treated by menadione to elicit oxidative stress. Roots were fed with 13C labelled glucose and the redistribution of isotope was determined in order to study carbon flow. The label redistribution through many pathways such as glycolysis, the tricarboxylic acid (TCA) cycle and amino acid metabolism were reduced under oxidative stress. After menadione removal many of the stress-related changes reverted back to basal levels. Decreases in amounts of hexose phosphates, malate, 2-oxoglutarate, glutamate and aspartate were fully recovered or even increased to above the control level. However, some metabolites such as pentose phosphates and citrate did not recover but maintained their levels or even increased further. The alteration in label redistribution largely correlated with that in metabolite abundance. Glycolytic carbon flow reverted to the control level only 18 h after menadione removal although the TCA cycle and some amino acids such as aspartate and glutamate took longer to recover. Taken together, plant root metabolism was demonstrated to be able to overcome menadione-induced oxidative stress with the differential time period required by independent pathways suggestive of the involvement of pathway specific regulatory processes. The online version of this article (doi:10.1007/s11306-011-0296-1) contains supplementary material, which is available to authorized users.
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