Glucose and auxin signaling interaction in controlling Arabidopsis thaliana seedlings root growth and development.
Glucose and auxin signaling interaction in controlling Arabidopsis thaliana seedlings root growth and development.
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葡萄糖和生长素信号传导相互作用在控制拟南芥幼苗根生长和发育中。
DOI：
10.1371/journal.pone.0004502
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Laxmi A
中科院分区：
文献类型：
--
作者：
Mishra BS;Singh M;Aggrawal P;Laxmi A
植物根系生长发育具有很强的可塑性，能够适应多种环境条件。糖信号已被证明通过与赤霉素、细胞分裂素和脱落酸等植物激素相互作用来影响根的生长发育。生长素的信号和运输已被证明控制植物的根长、侧根数量、根毛和根的生长方向。增加葡萄糖浓度不仅可以控制根长、根毛和侧根数量，还可以调节根的生长方向。由于根的生长和发育也受生长素的控制，因此进行了全基因组转录谱分析，以了解葡萄糖和生长素反应途径之间的相互作用程度。在受IAA影响的604个基因中，仅葡萄糖就能转录调控376个基因(62%)。葡萄糖的存在也可以调节IAA诱导或抑制的几乎63%的基因的调控程度2倍或更多。有趣的是，葡萄糖可以影响IAA相关基因的诱导或抑制(35%)，即使葡萄糖本身对这些基因的转录没有显著影响。葡萄糖可以影响生长素生物合成丝兰基因家族成员、生长素转运蛋白PIN蛋白、受体TIR1以及参与生长素信号转导的AUX/IAA、GH3和SAUR等基因家族成员。拟南芥生长素受体TIR1及其响应突变体axr2、axr3和slr1不仅在葡萄糖诱导的根长、根毛伸长和侧根产量的变化中存在缺陷，而且从垂直方向加剧了葡萄糖诱导的根生长随机增加，表明葡萄糖对植物根的生长发育的影响是由生长素信号成分介导的。我们的研究结果表明，在变化的营养条件下，葡萄糖与生长素信号和运输机制相互作用，控制幼苗根的生长和发育。
Plant root growth and development is highly plastic and can adapt to many environmental conditions. Sugar signaling has been shown to affect root growth and development by interacting with phytohormones such as gibberellins, cytokinin and abscisic acid. Auxin signaling and transport has been earlier shown to be controlling plant root length, number of lateral roots, root hair and root growth direction. Increasing concentration of glucose not only controls root length, root hair and number of lateral roots but can also modulate root growth direction. Since root growth and development is also controlled by auxin, whole genome transcript profiling was done to find out the extent of interaction between glucose and auxin response pathways. Glucose alone could transcriptionally regulate 376 (62%) genes out of 604 genes affected by IAA. Presence of glucose could also modulate the extent of regulation 2 fold or more of almost 63% genes induced or repressed by IAA. Interestingly, glucose could affect induction or repression of IAA affected genes (35%) even if glucose alone had no significant effect on the transcription of these genes itself. Glucose could affect auxin biosynthetic YUCCA genes family members, auxin transporter PIN proteins, receptor TIR1 and members of a number of gene families including AUX/IAA, GH3 and SAUR involved in auxin signaling. Arabidopsis auxin receptor tir1 and response mutants, axr2, axr3 and slr1 not only display a defect in glucose induced change in root length, root hair elongation and lateral root production but also accentuate glucose induced increase in root growth randomization from vertical suggesting glucose effects on plant root growth and development are mediated by auxin signaling components. Our findings implicate an important role of the glucose interacting with auxin signaling and transport machinery to control seedling root growth and development in changing nutrient conditions.
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影响因子：
5.1
作者：
Dekkers, Bas J. W.;Schuurmans, Jolanda A. M. J.;Smeekens, Sjef C. M.
通讯作者：
Smeekens, Sjef C. M.
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