Specificity of root microbiomes in native‐grown Nicotiana attenuata and plant responses to UVB increase Deinococcus colonization
Specificity of root microbiomes in native‐grown Nicotiana attenuata and plant responses to UVB increase Deinococcus colonization
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本地生长的烟草根部微生物组的特异性和植物对 UVB 的反应增加了奇异球菌的定殖
DOI：
10.1111/mec.14049
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发表时间：
2017
影响因子：
4.9
通讯作者：
Baldwin IT
中科院分区：
文献类型：
--
作者：
Santhanam R;Kumar R;Weinhold A;Luu VT;Groten K;Baldwin IT
植物从它们发芽的土壤中招募微生物群落。对于大多数微生物类群，我们对招募过程及其影响因素的了解仍然有限。我们分析了可能影响根部微生物组结构的几个因素——自然种群地理位置的重要性、作为推定定植来源的本地种子微生物组以及植物对 UVB 暴露的反应对高丰度物种根部定植的影响。烟草减毒种子的微生物组是通过培养依赖和培养独立的方法确定的，而天然烟草的根微生物组是通过培养依赖和非培养的方法确定的。 使用 454-焦磷酸测序分析了来自五个不同地点的减毒种群。为了具体解决 UVB 光对奇球菌 (Deinococcus) 根部定植的影响，奇球菌是一种在 N 中大量存在且持续存在的属。 在使用合成细菌群落补充/不补充 UVB 的微观实验中，研究了 UVB 感知 (irUVR8) 和反应 (irCHAL) 受损的转基因系。种子微生物组分析表明N. 减毒种子是无菌的。土壤和根之间原生根细菌群落的α和β多样性存在显着差异，而位置仅对真菌有显着影响，但对细菌根群落没有显着影响。与未处理的植物相比，补充 UVB 后，野生型中异常球菌的根定殖增加，但在irUVR8和dirCHAL植物中减少。我们的结果表明 N. attenuata仅从土壤中招募核心根微生物群，真菌根定植的选择性低于细菌定植。奇异球菌在根部的定植取决于植物对 UVB 的反应。
Plants recruit microbial communities from the soil in which they germinate. Our understanding of the recruitment process and the factors affecting it is still limited for most microbial taxa. We analysed several factors potentially affecting root microbiome structure – the importance of geographic location of natural populations, the microbiome of native seeds as putative source of colonization and the effect of a plant's response to UVB exposure on root colonization of highly abundant species. The microbiome ofNicotiana attenuataseeds was determined by a culture‐dependent and culture‐independent approach, and the root microbiome of naturalN. attenuatapopulations from five different locations was analysed using 454‐pyrosequencing. To specifically address the influence of UVB light on root colonization byDeinococcus, a genus abundant and consistently present inN. attenuataroots, transgenic lines impaired in UVB perception (irUVR8) and response (irCHAL) were investigated in a microcosm experiment with/without UVB supplementation using a synthetic bacterial community. The seed microbiome analysis indicated thatN. attenuataseeds are sterile. Alpha and beta diversities of native root bacterial communities differed significantly between soil and root, while location had only a significant effect on the fungal but not the bacterial root communities. With UVB supplementation, root colonization ofDeinococcusincreased in wild type, but decreased inirUVR8andirCHALplants compared to nontreated plants. Our results suggest thatN. attenuatarecruits a core root microbiome exclusively from soil, with fungal root colonization being less selective than bacterial colonization. Root colonization byDeinococcusdepends on the plant's response to UVB.
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影响因子：
16.6
作者：
Hacquard S;Kracher B;Hiruma K;Münch PC;Garrido-Oter R;Thon MR;Weimann A;Damm U;Dallery JF;Hainaut M;Henrissat B;Lespinet O;Sacristán S;Ver Loren van Themaat E;Kemen E;McHardy AC;Schulze-Lefert P;O'Connell RJ
通讯作者：
O'Connell RJ
DOI：
10.1073/pnas.1505765112
发表时间：
2015-09-08
影响因子：
11.1
作者：
Santhanam, Rakesh;Van Thi Luu;Baldwin, Ian T.
通讯作者：
Baldwin, Ian T.
影响因子：
8.8
作者：
Wagner MR;Lundberg DS;Coleman-Derr D;Tringe SG;Dangl JL;Mitchell-Olds T
通讯作者：
Mitchell-Olds T
正信号和负信号调节火后一年生烟草的发芽
DOI：
--
发表时间：
1999
期刊：
影响因子：
--
作者：
C. Preston;I. Baldwin
通讯作者：
I. Baldwin
DOI：
10.1073/pnas.1503106112
发表时间：
2015-07-28
影响因子：
11.1
作者：
Li, Dapeng;Baldwin, Ian T.;Gaquerel, Emmanuel
通讯作者：
Gaquerel, Emmanuel