The diagnostic accuracy of direct agglutination test for serodiagnosis of human visceral leishmaniasis: a systematic review with meta-analysis.
The diagnostic accuracy of direct agglutination test for serodiagnosis of human visceral leishmaniasis: a systematic review with meta-analysis.
复制标题
DOI：
10.1186/s12879-020-05558-7
复制
发表时间：
2020-12-11
影响因子：
3.7
通讯作者：
Mahmoudi S
中科院分区：
文献类型：
--
作者：
Mohebali M;Keshavarz H;Shirmohammad S;Akhoundi B;Borjian A;Hassanpour G;Mamishi S;Mahmoudi S
近三十年来，直接凝集试验(DAT)作为一种简便、准确、可靠的方法被广泛用于内脏利什曼病(VL)的血清学诊断。本研究是一项系统的综述和荟萃分析，旨在评价DAT在人类VL血清诊断中的准确性。检索了2004年12月至2019年4月期间的电子数据库，包括MEDLINE(通过PubMed)、Scope us、Web of Science、SID和Mag伊朗(两个波斯科学搜索引擎)。我们确定了DAT诊断人类VL的综合敏感度和特异度，计算了阳性和阴性似然比(LR+和LR-)，并构建了符合条件的研究的受试者工作特征(ROC)曲线参数。在上述电子数据库中确定的2928条记录中，在检查了参考文献清单后，有24条符合纳入标准，并参加了系统审查，其中有20条记录符合荟萃分析条件。DAT的综合敏感性和特异性分别为96%[95%CI，92~98]和95%[CI 95%86~99]。阳性试验的似然比(LR+)为2 1[CI95%，6.6-66.5]，阴性试验的似然比(LR−)为0.0 4[(CI95%，0.0 2-0.0 8])。综合估计DAT的诊断优势比很高[467(CI95%，114-1912])。我们发现汇总接收器工作特性曲线(SROC)位于曲线的左上角附近，曲线下面积(AUC)为0.98(95%可信区间为0.97~0.99)。参考我们的分析，我们认为DAT可以被认为是一种灵敏度和特异度都很高的VL血清学诊断工具。DAT是一种简便、准确、有效的血清学检测方法，可用于人类VL的血清学诊断，特别是在流行地区。
Direct agglutination test (DAT) as a simple, accurate and reliable method, has been widely used for serodiagnosis of visceral leishmaniasis (VL) during the last three decades. The present study is a systematic review and meta-analysis to evaluate the diagnostic accuracy of DAT for serodiagnosis of human VL. Electronic databases, including MEDLINE (via PubMed), SCOPUS, Web of Science, SID and Mag Iran (two Persian scientific search engines) were searched from December 2004 to April 2019. We determined the pooled sensitivity and specificity rates of DAT for the diagnosis of human VL, calculated positive and negative likelihood ratios (LR+ and LR-), and constructed summary receiver operating characteristic (ROC) curves parameters across the eligible studies. Of the 2928 records identified in the mentioned electronic databases and after examining reference lists of articles, 24 articles met inclusion criteria and were enrolled in the systematic review and out of them 20 records qualified for meta-analysis. The pooled sensitivity and specificity rates of DAT was 96% [95% CI, 92–98] and 95% [CI95% 86–99], respectively. The likelihood ratio of a positive test (LR+) was found to be 21 [CI95%, 6.6–66.5] and the likelihood ratio of a negative test (LR−) was found to be 0.04 [(CI95%, 0.02–0.08]. The combined estimate of the diagnostic odds ratio for DAT was high [467 (CI95%, 114–1912]). We found that the summary receiver operating characteristic curve (SROC) is positioned near the upper left corner of the curve and the area under curve (AUC) was 0.98 (95% CI, 0.97 to 0.99). Referring to our analysis, we determined that DAT can be considered as a valuable tool for the serodiagnosis of human VL with high sensitivity and specificity. As DAT is a simple, accurate and efficient serological test, it can be recommended for serodiagnosis of human VL particularly in endemic areas.
登录
查看更多内容
影响因子：
3.8
作者：
Bangert M;Flores-Chávez MD;Llanes-Acevedo IP;Arcones C;Chicharro C;García E;Ortega S;Nieto J;Cruz I
通讯作者：
Cruz I
影响因子：
3.3
作者：
Barbosa Junior, Walter Lins;Ramos de Araujo, Paulo Sergio;Medeiros, Zulma
通讯作者：
Medeiros, Zulma
影响因子：
--
作者：
Gavgani, A.S. Mazloumi;Ghazanchaei, A.;Davies, C.R.
通讯作者：
Davies, C.R.
影响因子：
7.2
作者：
Deeks, JJ;Macaskill, P;Irwig, L
通讯作者：
Irwig, L
DOI：
10.1016/0035-9203(90)90141-z
发表时间：
1990-09-01
影响因子：
2.2
作者：
HAILU, A
通讯作者：
HAILU, A