Construction of an Effective Protein Expression System Using the tpl Promoter in Escherichia coli
Construction of an Effective Protein Expression System Using the tpl Promoter in Escherichia coli
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利用 tpl 启动子在大肠杆菌中构建有效的蛋白质表达系统
DOI：
10.1007/s10529-005-0216-4
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发表时间：
2005
影响因子：
2.7
通讯作者：
H. Kumagai
中科院分区：
文献类型：
--
作者：
T. Koyanagi;T. Katayama;Ai Hirao;Hideyuki Suzuki;H. Kumagai
利用草欧文氏菌酪氨酸苯酚裂解酶(TPL)基因启动子，构建了一种高效的蛋白表达系统。这个系统涉及突变形式的TyrR蛋白和TutB蛋白，前者激活TPL的能力增强，后者具有转运L酪氨酸(TPL的诱导剂)的能力。该系统的最高表达水平是TAC系统的两倍多，但低于T7系统的表达水平，如乳胶报告分子实验和SDS-聚丙烯酰胺凝胶电泳法。
An effective protein expression system was constructed in Escherichia coli using the promoter of the tyrosine phenol-lyase (tpl) gene of Erwinia herbicola. This system involves a mutant form of the TyrR protein with an enhanced ability to activate tpl and the TutB protein with an ability to transport L-tyrosine (an inducer of Tpl). The highest expression level obtained for this system was more than twice that obtained for the tac system, although it was lower than the level obtained for the T7 system, as revealed with the lac-reporter assay and SDS-polyacrylamide gel electrophoresis.
影响因子：
4.1
作者：
Koyanagi, T;Katayama, T;Kumagai, H
通讯作者：
Kumagai, H