A BTP1 prophage gene present in invasive non-typhoidal Salmonella determines composition and length of the O-antigen of the lipopolysaccharide.
A BTP1 prophage gene present in invasive non-typhoidal Salmonella determines composition and length of the O-antigen of the lipopolysaccharide.
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DOI：
10.1111/mmi.12933
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发表时间：
2015-04
影响因子：
3.6
通讯作者：
van der Woude MW
中科院分区：
文献类型：
--
作者：
Kintz E;Davies MR;Hammarlöf DL;Canals R;Hinton JC;van der Woude MW
鼠伤寒沙门氏菌分离株D23580代表了最近鉴定的侵袭性非伤寒沙门氏菌（iNTS）的ST 313谱系。 这个谱系和其他非iNTS S的区别之一是。鼠伤寒分离株中存在原噬菌体BTP 1。该原噬菌体编码与0-抗原修饰有关的gtrC基因。GtrCBTP 1对于维持分离株D23580中的O-抗原长度至关重要，因为gtrBTP 1突变体产生短O-抗原。这种表型可以由gtrCBTP 1或非常密切相关的gtrC基因互补。gtrBTP 1突变体的短O-抗原也通过缺失D23580染色体中的BTP 1噬菌体尾钉基因来补偿。该尾刺蛋白具有推定的内鼠李糖苷酶结构域，因此可以介导O-抗原切割。与许多其他gtr操纵子的表达相反，gtrCBTP 1基因的表达不受相位变化的影响，转录分析表明gtrC在多种条件下产生。此外，GtrCBTP 1表达是必要的，足以提供保护，防止BTP 1噬菌体感染的其他敏感菌株。这些数据与GtrCBTP 1介导溶原性D23580中BTP 1噬菌体O-抗原受体的修饰的模型一致，从而防止自身和使用相同O-抗原共受体的其他噬菌体的重复感染。
Salmonella Typhimurium isolate D23580 represents a recently identified ST313 lineage of invasive non-typhoidal Salmonellae (iNTS). One of the differences between this lineage and other non-iNTS S. Typhimurium isolates is the presence of prophage BTP1. This prophage encodes a gtrC gene, implicated in O-antigen modification. GtrCBTP1 is essential for maintaining O-antigen length in isolate D23580, since a gtrBTP1 mutant yields a short O-antigen. This phenotype can be complemented by gtrCBTP1 or very closely related gtrC genes. The short O-antigen of the gtrBTP1 mutant was also compensated by deletion of the BTP1 phage tailspike gene in the D23580 chromosome. This tailspike protein has a putative endorhamnosidase domain and thus may mediate O-antigen cleavage. Expression of the gtrCBTP1 gene is, in contrast to expression of many other gtr operons, not subject to phase variation and transcriptional analysis suggests that gtrC is produced under a variety of conditions. Additionally, GtrCBTP1 expression is necessary and sufficient to provide protection against BTP1 phage infection of an otherwise susceptible strain. These data are consistent with a model in which GtrCBTP1 mediates modification of the BTP1 phage O-antigen receptor in lysogenic D23580, and thereby prevents superinfection by itself and other phage that uses the same O-antigen co-receptor.
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影响因子：
30.8
作者：
Holt, Kathryn E.;Parkhill, Julian;Mazzoni, Camila J.;Roumagnac, Philippe;Weill, Francois-Xavier;Goodhead, Ian;Rance, Richard;Baker, Stephen;Maskell, Duncan J.;Wain, John;Dolecek, Christiane;Achtman, Mark;Dougan, Gordon
通讯作者：
Dougan, Gordon
影响因子：
5.6
作者：
Steinbacher, S;Miller, S;Huber, R
通讯作者：
Huber, R
影响因子：
3.5
作者：
CHEREPANOV, PP;WACKERNAGEL, W
通讯作者：
WACKERNAGEL, W
影响因子：
5.8
作者：
Möller, S;Croning, MDR;Apweiler, R
通讯作者：
Apweiler, R
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL