Investigations on the role of a lysozyme from the malaria vector Anopheles dirus during malaria parasite development.
Investigations on the role of a lysozyme from the malaria vector Anopheles dirus during malaria parasite development.
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DOI：
10.1016/j.dci.2011.06.010
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发表时间：
2012-01
影响因子：
2.9
通讯作者：
Paskewitz, Susan M.
中科院分区：
文献类型：
--
作者：
Lapcharoen, Parichat;Komalamisra, Narumon;Rongsriyam, Yupha;Wangsuphachart, Voranuch;Dekumyoy, Paron;Prachumsri, Jetsumon;Kajla, Mayur K.;Paskewitz, Susan M.
通过cDNA末端快速扩增-聚合酶链反应（RACE-PCR）从大劣按蚊A（双翅目：蚊科）的雌性获得编码溶菌酶的cDNA。623 bp的溶菌酶（AdLys c-1）cDNA编码120个氨基酸的成熟蛋白，预测分子量为13.4 kDa，理论pI为8.45。六个半胱氨酸残基和一个潜在的钙结合基序，是目前在AdLys C-1是高度保守的相对于在其他昆虫中发现的C-型溶菌酶。RT-PCR分析的AdLys c-1转录本显示其存在于高水平的唾液腺无论是在幼虫和成人阶段，并在幼虫盲肠。进行dsRNA介导的基因敲低实验以检查这种溶菌酶在伯氏疟原虫感染期间的潜在作用。与对照dsGFP注射的蚊子相比，AdLys c-1的沉默导致卵囊数量的显著减少。
A cDNA encoding a lysozyme was obtained by rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR) from females of the malaria vector Anopheles dirus A (Diptera: Culicidae). The 623 bp lysozyme (AdLys c-1) cDNA encodes the 120 amino acid mature protein with a predicted molecular mass of 13.4 kDa and theoretical pI of 8.45. Six cysteine residues and a potential calcium binding motif that are present in AdLys c-1 are highly conserved relative to those of c-type lysozymes found in other insects. RT-PCR analysis of the AdLys c-1 transcript revealed its presence at high levels in the salivary glands both in larval and adult stages and in the larval caecum. dsRNA mediated gene knockdown experiments were conducted to examine the potential role of this lysozyme during Plasmodium berghei infection. Silencing of AdLys c-1 resulted in a significant reduction in the number of oocysts as compared to control dsGFP injected mosquitoes.
DOI：
10.4269/ajtmh.1996.54.214
发表时间：
1996-02-01
影响因子：
3.3
作者：
Pumpuni, CB;DeMaio, J;Beier, JC
通讯作者：
Beier, JC
影响因子：
2.1
作者：
PUMPUNI, CB;BEIER, MS;DAVIS, JR
通讯作者：
DAVIS, JR