Transcription analysis on response of swine lung to H1N1 swine influenza virus.
Transcription analysis on response of swine lung to H1N1 swine influenza virus.
复制标题
猪肺对H1N1猪流感病毒反应的转录分析
DOI：
10.1186/1471-2164-12-398
复制
发表时间：
2011-08-08
期刊：
影响因子：
4.4
通讯作者：
Jin M
中科院分区：
文献类型：
--
作者：
Li Y;Zhou H;Wen Z;Wu S;Huang C;Jia G;Chen H;Jin M
【摘要】：背景猪流感作为一种温和、高度传染性的呼吸道疾病,常损害猪的先天免疫系统,增加继发感染的易感性,导致猪的发病率和死亡率相当高。然而，猪对猪流感病毒感染的系统宿主反应仍然很大程度上未知。为了探究这一问题，我们进行了时程基因表达谱分析，以全面分析甲型H1N1猪流感病毒在猪体内引起的整体宿主反应。结果在甲型H1N1猪流感病毒感染的早期，猪出现轻微的呼吸道症状和病理变化。鉴定出总共 268 个在接种后表现出差异表达 (DE) 的猪基因，并与感染后第 3 天 (PID) 的对照进行比较（倍数变化 ≥ 2，p < 0.05）。 DE基因涉及许多重要的功能类别，主要包括信号转导、免疫反应、炎症反应、细胞粘附和细胞间信号传导。值得注意的是，与免疫和炎症反应相关的基因表现出高度过度表达。通过通路分析发现，重要通路主要涉及细胞粘附分子、细胞因子-细胞因子受体相互作用、Toll样受体信号通路和MAPK信号通路，提示宿主采取不同策略激活这些通路，从而在早期预防病毒感染。然而，在 PID 7 上，DE 基因的主要功能类别包括信号转导、代谢、转录、发育和运输。此外，最重要的通路转向了 PPAR 信号通路以及补体和凝血级联，表明宿主可能开始通过抗炎功能修复过度的组织损伤。 PID 7 的这些结果表明，通过激活这些基因簇，宿主可以进行有益的周转，以防止过度炎症损伤并恢复正常状态。结论这项研究显示了猪的靶器官如何响应 H1N1 猪流感病毒感染。观察到的基因表达谱有助于筛选降低猪流感病毒流行的潜在宿主因子，并进一步了解与猪感染H1N1流感相关的分子发病机制。
BackgroundAs a mild, highly contagious, respiratory disease, swine influenza always damages the innate immune systems, and increases susceptibility to secondary infections which results in considerable morbidity and mortality in pigs. Nevertheless, the systematical host response of pigs to swine influenza virus infection remains largely unknown. To explore it, a time-course gene expression profiling was performed for comprehensive analysis of the global host response induced by H1N1 swine influenza virus in pigs.ResultsAt the early stage of H1N1 swine virus infection, pigs were suffering mild respiratory symptoms and pathological changes. A total of 268 porcine genes showing differential expression (DE) after inoculation were identified to compare with the controls on day 3 post infection (PID) (Fold change ≥ 2, p < 0.05). The DE genes were involved in many vital functional classes, mainly including signal transduction, immune response, inflammatory response, cell adhesion and cell-cell signalling. Noticeably, the genes associated with immune and inflammatory response showed highly overexpressed. Through the pathway analysis, the significant pathways mainly concerned with Cell adhesion molecules, Cytokine-cytokine receptor interaction, Toll-like receptor signaling pathway and MAPK signaling pathway, suggesting that the host took different strategies to activate these pathways so as to prevent virus infections at the early stage. However, on PID 7, the predominant function classes of DE genes included signal transduction, metabolism, transcription, development and transport. Furthermore, the most significant pathways switched to PPAR signaling pathway and complement and coagulation cascades, showing that the host might start to repair excessive tissue damage by anti-inflammatory functions. These results on PID 7 demonstrated beneficial turnover for host to prevent excessive inflammatory damage and recover the normal state by activating these clusters of genes.ConclusionsThis study shows how the target organ responds to H1N1 swine influenza virus infection in pigs. The observed gene expression profile could help to screen the potential host agents for reducing the prevalence of swine influenza virus and further understand the molecular pathogenesis associated with H1N1 infection in pigs.
登录
查看更多内容
在B淋巴细胞缺陷小鼠中对致命流感病毒感染的抗性和恢复。
影响因子：
15.3
作者：
Graham, M B;Braciale, T J
通讯作者：
Braciale, T J
影响因子：
3.8
作者：
Brown, IH;Ludwig, S;Alexander, DJ
通讯作者：
Alexander, DJ
影响因子：
3.4
作者：
Chen, Chao-Hsuan;Zhang, Xing-Quan;Lo, Chih-Wei;Liu, Pei-Feng;Liu, Yu-Tsueng;Gallo, Richard L.;Hsieh, Ming-Fa;Schooley, Robert T.;Huang, Chun-Ming
通讯作者：
Huang, Chun-Ming
影响因子：
4.4
作者：
Herold, Susanne;von Wulffen, Werner;Lohmeyer, Juergen
通讯作者：
Lohmeyer, Juergen
影响因子：
4.4
作者：
Durbin, JE;Fernandez-Sesma, A;Levy, DE
通讯作者：
Levy, DE