Duganella albus sp. nov., Duganella aquatilis sp. nov., Duganella pernnla sp. nov. and Duganella levis sp. nov., isolated from subtropical streams in China.
Duganella albus sp. nov., Duganella aquatilis sp. nov., Duganella pernnla sp. nov. and Duganella levis sp. nov., isolated from subtropical streams in China.
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杜加内拉阿布斯 sp。
DOI：
10.1099/ijsem.0.004234
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发表时间：
2020-05
影响因子：
2.8
通讯作者：
Xu Meiying
中科院分区：
文献类型：
--
作者：
Lu Huibin;Deng Tongchu;Liu Feifei;Wang Yonghong;Yang Xunan;Xu Meiying
从中国亚热带河流中分离到6株革兰氏阴性、过氧化氢酶和氧化酶阳性、杆状和运动性菌株(FT9WT、FT25W、FT26WT、FT109WT、FT134W和CY42WT)。基于16S rRNA基因序列的比较表明，这6个菌株与该科内其他物种的相似性不到98.1%
草杆菌科
并在系统发育树上形成了两个不同的分支。FT9WT株与FT25W株、FT109WT株与FT134W株16S rRNA基因序列同源性均为99.7%。FT9WT、FT25W、FT26WT、FT109WT、FT134W和CY42WT的基因组大小分别为6.45、6.45、6.54、6.43、6.52和6.74Mbp，G+C含量分别为64.0、64.0、63.8、63.2、63.2和62.5%。除FT9WT株与FT25W株之间、FT109WT株与FT134W株之间、FT26WT株与FT9WT株和FT25W株之间分别为99.9%、98.2%、95.0%和95.1%外，其余6个菌株与其他近缘种的平均核苷酸(ANI)值均小于93.9%。而FT26WT株与FT9WT株和FT25W株的16S rRNA基因序列相似性分别为98.3%和98.2%。经鉴定，这6株菌的呼吸性苯二酚为Q-8。主要脂肪酸为C16：1
ω7c、C16：0和C12：0。主要的极性脂包括磷脂酰乙醇胺和磷脂酰甘油。综合表型、生化、基因分型和ANI数据，FT9WT和FT25W与FT109WT和FT134W可能分别属于同一种。虽然FT26WT菌株与FT9WT和FT25W菌株之间的ANI值都位于物种划分的过渡区，但它们之间的差异表明FT26WT菌株可能代表了一个独立的新物种。根据92个核心基因的串联构建的系统发育树显示，这6个菌株与
甘蔗杜氏杆菌
SAC-22T和
根杜氏杜氏杆菌
KCTC 22382T，支持这6株菌属于该属。
杜加内拉
。命名为白色杜加内氏菌Duganella albus sp.11月(模式菌株FT9WT=GDMCC 1.1637T=KACC 21313T)，水生杜氏杆菌(Duganella Aquatilis sp.)11月(模式菌株FT26WT=GDMCC 1.1641T=KACC21315T)，Pernnla Duganella sp.11月(模式菌株FT109WT=GDMCC 1.1688T=KACC21480T)和Duganella Levis sp.11月模式菌株CY42WT=GDMCC 1.1673T=KACC21465T。
Six Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile strains (FT9WT, FT25W, FT26WT, FT109WT, FT134W and CY42WT) were isolated from subtropical streams in China. Comparisons based on 16S rRNA gene sequences showed that the six strains shared similarities of less than 98.1 % with other species within the family
Oxalobacteraceae
and formed two separately distinct clades in phylogenetic trees. The 16S rRNA gene sequence similarities between strains FT9WT and FT25W, and between strains FT109WT and FT134W were both 99.7 %. The genome sizes of strains FT9WT, FT25W, FT26WT, FT109WT, FT134W and CY42WT were 6.45, 6.45, 6.54, 6.43, 6.52 and 6.74 Mbp with G+C contents of 64.0, 64.0, 63.8, 63.2, 63.2 and 62.5 %, respectively. The calculated pairwise average nucleotide (ANI) values among the six strains and other related species were less than 93.9 %, except that the values were 99.9 % between strains FT9WT and FT25W, 98.2 % between strains FT109WT and FT134W, and 95.0 and 95.1 % between strain FT26WT and strains FT9WT and FT25W, respectively. However, strain FT26WT shared 16S rRNA gene sequence similarities of only 98.3 and 98.2 % with FT9WT and FT25W, respectively. The respiratory quinone of the six strains was determined to be Q-8. The major fatty acids were C16 : 1
ω7c, C16 : 0 and C12 : 0. The predominant polar lipids included phosphatidylethanolamine and phosphatidylglycerol. Considering the phenotypic, biochemical, genotypic and ANI data, strains FT9WT and FT25W, and FT109WT and FT134W may belong to the same species, respectively. Although the pairwise ANI values between strain FT26WT and each of strains FT9WT and FT25W were located in the transition region of species demarcation, the dissimilarities among them indicated that strain FT26WT could represent an independent novel species. The reconstructed phylogenomic tree based on a concatenation of 92 core genes showed that the six strains clustered closely with
Duganella sacchari
Sac-22T and
Duganella radicis
KCTC 22382T, and supported that these six strains belong to the genus
Duganella
. The names Duganella albus sp. nov. (type strain FT9WT=GDMCC 1.1637T=KACC 21313T), Duganella aquatilis sp. nov. (type strain FT26WT=GDMCC 1.1641T=KACC 21315T), Duganella pernnla sp. nov. (type strain FT109WT=GDMCC 1.1688T=KACC 21480T) and Duganella levis sp. nov. (type strain CY42WT=GDMCC 1.1673T=KACC 21465T) are proposed.
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DOI：
10.1099/ijsem.0.004097
发表时间：
2020-01-01
影响因子：
2.8
作者：
Lu, Huibin;Deng, Tongchu;Xu, Meiying
通讯作者：
Xu, Meiying
影响因子：
2.1
作者：
B. Tindall
通讯作者：
B. Tindall
影响因子：
1.5
作者：
A. Ventosa;E. Quesada;F. Rodríguez-Valera;F. Ruiz-Berraquero;A. Ramos‐Cormenzana
通讯作者：
A. Ventosa;E. Quesada;F. Rodríguez-Valera;F. Ruiz-Berraquero;A. Ramos‐Cormenzana
DOI：
10.1099/00207713-38-4-358
发表时间：
1988-10-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
KUYKENDALL, LD;ROY, MA;DEVINE, TE
通讯作者：
DEVINE, TE
影响因子：
3
作者：
Meier-Kolthoff JP;Auch AF;Klenk HP;Göker M
通讯作者：
Göker M