Early emergence of OXA-181-producing Escherichia coli ST410 in China.
Early emergence of OXA-181-producing Escherichia coli ST410 in China.
复制标题
产OXA-181大肠杆菌ST410在中国较早出现。
DOI：
10.1016/j.jgar.2018.06.017
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发表时间：
2018-12
期刊：
影响因子：
--
通讯作者：
Liu Hong Min
中科院分区：
文献类型：
--
作者：
Qin Shangshang;Cheng Jing;Wang Ping;Feng Xianju;Liu Hong Min
目的调查河南省产oxa -48样肠杆菌科的流行情况和特征。方法对2013年1月至2016年12月临床分离的339株碳青霉烯类非敏感肠杆菌科菌株[亚胺培南或美罗培南最低抑菌浓度（MIC）为bb0.1 μg/mL]进行PCR和测序，检测其是否存在blaoxa -48样基因。采用MIC法检测对各种抗菌药的敏感性。采用多位点序列分型（MLST）进行细菌基因分型。质粒pEC21-OXA-181通过电穿孔转化到大肠杆菌h5α中，使用Illumina MiSeq平台进行测序，随后进行注释和遗传分析。结果339株碳青霉烯非敏感肠杆菌科分离株中，仅有1株（0.3%）为E。属于ST410的大肠杆菌EC21对blaoxa -181 （blaoxa -48的变体）呈阳性。这oxa - 181 producinge。从无国外旅行史的患者中恢复的大肠杆菌，比首次报告的blaoxa -181阳性更早获得。大肠杆菌（WCHEC14828）质粒分析结果显示，blaoxa -181与喹诺酮类耐药基因qnrs1在51 kb的incx3型质粒上由一个is26侧翼的复合转座子携带。结论这些发现提示了oxa -181产生基因的出现。进入中国的时间比预想的要早。blaoxa -181基因与广泛传播的潜在地方病有关。该基因由IncX3质粒携带，IncX3质粒是ndm型碳青霉烯酶的常见传播载体，可能促进blaoxa -181在中国肠杆菌科的进一步传播。
ObjectivesThe aim of this study was to investigate the prevalence and characterisation of OXA-48-like-producing Enterobacteriaceae in Henan Province, China.MethodsA total of 339 carbapenem-non-susceptible clinical Enterobacteriaceae isolates [imipenem or meropenem minimum inhibitory concentration (MIC) of >1 μg/mL] obtained between January 2013 and December 2016 were screened for the presence of theblaOXA-48-likegene by PCR and sequencing. Antimicrobial susceptibility to various antimicrobials agents was examined by MIC testing. Multilocus sequence typing (MLST) was performed for bacterial genotyping. The plasmid pEC21-OXA-181 was transformed intoEscherichia coliDH5α by electroporation and was sequenced using an Illumina MiSeq platform, followed by subsequent annotation and genetic analysis.ResultsAmong the 339 carbapenem-non-susceptible Enterobacteriaceae isolates, only one (0.3%)E. colistrain EC21, belonging to ST410, was positive forblaOXA-181, a variant ofblaOXA-48. This OXA-181-producingE. coli, recovered from a patient without a history of foreign travel, was obtained earlier than the first reportedblaOXA-181-positiveE. coli(WCHEC14828) in Sichuan Province, China. Plasmid analysis revealed thatblaOXA-181together with the quinolone resistance geneqnrS1was carried by an IS26-flanked composite transposon on a 51-kb IncX3-type plasmid.ConclusionsThese findings indicate the emergence of OXA-181-producingE. coliin China earlier than previously thought. TheblaOXA-181gene is associated with the widely disseminated potentially endemicE. coliST410 clone and is carried by an IncX3 plasmid, a common vehicle for spreading NDM-type carbapenemases, which might promote the further dissemination ofblaOXA-181among the Enterobacteriaceae in China.
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影响因子：
4.9
作者：
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通讯作者：
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DOI：
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影响因子：
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作者：
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作者：
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DOI：
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发表时间：
2017-08
期刊：
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
影响因子：
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作者：
N. S. Uwaezuoke;N. Kieffer;K. Iregbu;P. Nordmann
通讯作者：
N. S. Uwaezuoke;N. Kieffer;K. Iregbu;P. Nordmann