Brown adipose tissue monocytes support tissue expansion.
Brown adipose tissue monocytes support tissue expansion.
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DOI：
10.1038/s41467-021-25616-1
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发表时间：
2021-09-06
影响因子：
16.6
通讯作者：
Ivanov S
中科院分区：
文献类型：
--
作者：
Gallerand A;Stunault MI;Merlin J;Luehmann HP;Sultan DH;Firulyova MM;Magnone V;Khedher N;Jalil A;Dolfi B;Castiglione A;Dumont A;Ayrault M;Vaillant N;Gilleron J;Barbry P;Dombrowicz D;Mack M;Masson D;Bertero T;Becher B;Williams JW;Zaitsev K;Liu Y;Guinamard RR;Yvan-Charvet L;Ivanov S
单核细胞是单核吞噬系统的一部分。单核细胞在炎症条件下发挥核心作用，更好地了解它们的动力学可能会打开治疗的机会。在本研究中，我们重点研究了组织重塑过程中单核细胞对棕色脂肪组织（BAT）功能的影响。BAT免疫细胞的单细胞RNA测序分析揭示了单核细胞和巨噬细胞群体的巨大多样性。命运定位实验表明，BAT巨噬细胞池需要不断地从单核细胞中补充。利用BAT扩增的遗传模型，我们发现棕色脂肪单核细胞数量在这种情况下选择性地增加。使用ccr2结合放射性示踪剂和正电子发射断层扫描证实了这一观察结果。重要的是，与他们的组织招募一致，血液单核细胞计数减少，而骨髓造血不受影响。单核细胞消耗阻止棕色脂肪组织扩张并改变其结构。BAT扩展严格要求Podoplanin参与。总之，这些数据重新定义了BAT中免疫细胞的多样性，并强调了单核细胞募集在组织重塑中的作用。脂肪组织由许多脂肪细胞和包括免疫细胞在内的许多其他细胞组成。在这里，作者使用小鼠棕色脂肪组织免疫细胞的单细胞测序，描述了多个巨噬细胞和单核细胞亚群，并表明单核细胞有助于棕色脂肪组织的扩张。
Monocytes are part of the mononuclear phagocytic system. Monocytes play a central role during inflammatory conditions and a better understanding of their dynamics might open therapeutic opportunities. In the present study, we focused on the characterization and impact of monocytes on brown adipose tissue (BAT) functions during tissue remodeling. Single-cell RNA sequencing analysis of BAT immune cells uncovered a large diversity in monocyte and macrophage populations. Fate-mapping experiments demonstrated that the BAT macrophage pool requires constant replenishment from monocytes. Using a genetic model of BAT expansion, we found that brown fat monocyte numbers were selectively increased in this scenario. This observation was confirmed using a CCR2-binding radiotracer and positron emission tomography. Importantly, in line with their tissue recruitment, blood monocyte counts were decreased while bone marrow hematopoiesis was not affected. Monocyte depletion prevented brown adipose tissue expansion and altered its architecture. Podoplanin engagement is strictly required for BAT expansion. Together, these data redefine the diversity of immune cells in the BAT and emphasize the role of monocyte recruitment for tissue remodeling. Adipose tissue is composed of a number of adipocytes and a number of other cells including immune cells. Here the authors use single-cell sequencing of murine brown adipose tissue immune cells and describe multiple macrophage and monocyte subsets and show that monocytes contribute to brown adipose tissue expansion.
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DOI：
10.1084/jem.20160800
发表时间：
2016-10-17
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Chong SZ;Evrard M;Devi S;Chen J;Lim JY;See P;Zhang Y;Adrover JM;Lee B;Tan L;Li JL;Liong KH;Phua C;Balachander A;Boey A;Liebl D;Tan SM;Chan JK;Balabanian K;Harris JE;Bianchini M;Weber C;Duchene J;Lum J;Poidinger M;Chen Q;Rénia L;Wang CI;Larbi A;Randolph GJ;Weninger W;Looney MR;Krummel MF;Biswas SK;Ginhoux F;Hidalgo A;Bachelerie F;Ng LG
通讯作者：
Ng LG
巨噬细胞的局部扩散有助于与肥胖相关的脂肪组织炎症。
影响因子：
29
作者：
Amano SU;Cohen JL;Vangala P;Tencerova M;Nicoloro SM;Yawe JC;Shen Y;Czech MP;Aouadi M
通讯作者：
Aouadi M
影响因子：
32.4
作者：
Croxford, Andrew L.;Lanzinger, Margit;Becher, Burkhard
通讯作者：
Becher, Burkhard
影响因子：
30.5
作者：
通讯作者：
--
影响因子：
9
作者：
Abdullahi A;Auger C;Stanojcic M;Patsouris D;Parousis A;Epelman S;Jeschke MG
通讯作者：
Jeschke MG