Removal of immune-stimulatory components from surfaces by plasma discharges
Removal of immune-stimulatory components from surfaces by plasma discharges
复制标题
通过等离子体放电去除表面的免疫刺激成分
作者：
M. Hasiwa;O. Kylián;T. Hartung;F. Rossi
当通过手术器械或植入物与生物体接触时，结合到表面上的免疫刺激微生物组分如脂多糖（LPS）、脂磷壁酸（LTA）和酵母聚糖会导致严重的问题。在最近的研究中，我们已经表明，可以通过间接检测方法直接在表面上检测不同的免疫刺激成分，使用人全血和单核细胞反应通过ELISA测量炎症介质释放（IL-1β）。关于热原物质的灭活，我们提出了一种基于应用在低温下工作的低压微波等离子体放电的方法。我们发现，LPS，LTA和酵母聚糖的免疫刺激能力的快速（10秒至几分钟）去除率。为了模拟细菌细胞壁，使用LPS与胞壁酰二肽的组合，并且炎症信号的降低速率与纯LPS没有差异。
Immune-stimulating microbiological components like lipopolysaccharide (LPS), lipoteichoic acid (LTA) and zymosan bound onto surfaces lead to severe problems when brought in contact with the organism via surgical instruments or implants. We have shown, in recent studies, that it is possible to detect different immune-stimulating components directly on the surface, via an indirect detection method, using human whole-blood and the monocyte reaction to measure the inflammatory mediator release (IL-1β) by ELISA. With regard to the inactivation of pyrogenic substances, we present a method based on the application of a low-pressure microwave plasma discharge working at low temperatures. We found a fast (10 s to a few minutes) removal rate of the immune-stimulating competence for LPS, LTA and zymosan. To mimic the bacterial cell-wall, LPS in combination with muramyl dipeptide was employed and the decreasing rate of the inflammatory signal did not differ from pure LPS.
免疫佐剂增强大鼠对内源性热原的发热反应。
DOI：
10.1152/jappl.1989.67.5.1734
发表时间：
1989
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
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作者：
Stitt,JT;Shimada,SG
通讯作者：
Shimada,SG