The zinc finger protein Zn72D and DEAD box helicase Belle interact and control maleless mRNA and protein levels.
The zinc finger protein Zn72D and DEAD box helicase Belle interact and control maleless mRNA and protein levels.
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DOI：
10.1186/1471-2199-10-33
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发表时间：
2009-04-22
影响因子：
--
通讯作者：
Panning B
中科院分区：
文献类型：
--
作者：
Worringer KA;Chu F;Panning B
雄性特异性致死(MSL)复合体富含在雄性果蝇细胞的单个X染色体上，其功能是上调X连锁基因的表达，并使X连锁基因的剂量与XX雌性果蝇的X连锁基因剂量相等。锌指蛋白Zn72D是无马来酸(MLE)转录本高效剪接所必需的，MLE转录本编码MSL复合体的一个重要亚基。在缺乏锌72D的情况下，MLE水平降低，结果是MSL复合体不再定位于X染色体，剂量补偿被破坏。为了了解锌72D功能的分子基础，我们鉴定了与锌72D相互作用的蛋白质。在与锌72D相关的几种蛋白质中，我们发现了死盒解旋酶Belle(Bel)。同时敲除ZN72D和BEL可恢复MSL复合体在X染色体上的定位和剂量补偿。MLE蛋白恢复到野生型的70%，尽管高效剪接的MLE转录本的水平仍然比野生型细胞低四倍。MLE蛋白产量相对于正确剪接的MLE mRNA数量的增加不能归因于MLE稳定性的改变。这些数据表明，Zn72D和Bel共同控制MLE剪接和蛋白质水平。因此，Zn72D和Bel可能是协调剪接和翻译调控的因子。
The Male Specific Lethal (MSL) complex is enriched on the single X chromosome in male Drosophila cells and functions to upregulate X-linked gene expression and equalize X-linked gene dosage with XX females. The zinc finger protein Zn72D is required for productive splicing of the maleless (mle) transcript, which encodes an essential subunit of the MSL complex. In the absence of Zn72D, MLE levels are decreased, and as a result, the MSL complex no longer localizes to the X chromosome and dosage compensation is disrupted. To understand the molecular basis of Zn72D function, we identified proteins that interact with Zn72D. Among several proteins that associate with Zn72D, we found the DEAD box helicase Belle (Bel). Simultaneous knockdown of Zn72D and bel restored MSL complex localization to the X chromosome and dosage compensation. MLE protein was restored to 70% of wild-type levels, although the level of productively spliced mle transcript was still four-fold lower than in wild-type cells. The increase in production of MLE protein relative to the amount of correctly spliced mle mRNA could not be attributed to an alteration in MLE stability. These data indicate that Zn72D and Bel work together to control mle splicing and protein levels. Thus Zn72D and Bel may be factors that coordinate splicing and translational regulation.
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DOI：
10.1083/jcb.200407173
发表时间：
2005-03-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kiesler E;Hase ME;Brodin D;Visa N
通讯作者：
Visa N
影响因子：
7
作者：
Chu, FX;Nusinow, DA;Burlingame, AL
通讯作者：
Burlingame, AL
影响因子：
56.9
作者：
Chuang, RY;Weaver, PL;Chang, TH
通讯作者：
Chang, TH
影响因子：
64.8
作者：
Hachet, O;Ephrussi, A
通讯作者：
Ephrussi, A
影响因子：
16.8
作者：
Burckin, T;Nagel, R;Ares, M
通讯作者：
Ares, M