CD40‐mediated stimulation of B1 and B2 cells: implication in autoantibody production in murine lupus
CD40‐mediated stimulation of B1 and B2 cells: implication in autoantibody production in murine lupus
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CD40 介导的 B1 和 B2 细胞刺激：对小鼠狼疮自身抗体产生的影响
DOI：
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发表时间：
1996
影响因子：
5.4
通讯作者：
T. Shirai
中科院分区：
文献类型：
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作者：
Y. Kaneko;S. Hirose;M. Abe;H. Yagita;K. Okumura;T. Shirai
B1细胞通常对T细胞非依赖性抗原表现出优先反应。为了了解B1细胞是否能对CD40介导的增殖和分化刺激做出反应，以及CD40介导的信号是否参与了B1细胞产生自身抗体的过程，我们比较了来自自身免疫倾向(NZB X NZW)F1小鼠的B1和B2细胞对我们新建立的激动型抗鼠CD40单抗(MAb)的反应。经该单抗刺激后，B1和B2细胞的增殖反应相似，细胞表面分子I-A、CD54、CD23、CD80和CD86的表达增加。IL-4联合刺激可显著增强B1和B2细胞的增殖反应以及IgG1和IgE抗体反应，而IL-5联合刺激仅增强B1细胞的增殖反应和IgM抗体反应。幼年(NZB X NZW)F1小鼠脾B1细胞自发产生大量的包括抗DNA抗体在内的IgM，且单独用抗CD40单抗刺激，或联合IL-4和IL-5刺激时，其水平升高。这些结果表明，自身免疫(NZB X NZW)F1小鼠脾B1细胞对CD40刺激的反应性与B2细胞相似，这可能是B1细胞自发产生自身抗体的一种强有力的调节机制。
B1 cells usually show preferential responses to T cell‐independent antigens. To ask whether B1 cells could respond to CD40‐mediated stimulation for proliferation and differentiation, and whether CD40‐mediated signals are involved in the production of autoantibodies by B1 cells, we compared responses to our newly established agonistic anti‐mouse CD40 monoclonal antibody (mAb) between B1 and B2 cells from autoimmune‐prone (NZB X NZW) F1 mice. Stimulation with this mAb induced a similar level of proliferative responses of both B1 and B2 cells, as well as an increase in expression of cell surface molecules I‐A, CD54, CD23, CD80, and CD86. While co‐stimulation with interleukin (IL)‐4 markedly augmented proliferative as well as IgG1 and IgE antibody responses of both B1 and B2 cells, co‐stimulation with IL‐5 augmented proliferative and IgM antibody responses of only B1 cells. Splenic B1, but not B2 cells from young (NZB X NZW) F1 mice spontaneously produced substantial amounts of IgM including IgM anti‐DNA antibodies, and the levels increased in case of stimulation with anti‐CD40 mAb alone, or to a greater extent with the mAb plus IL‐4 and IL‐5. Collectively, these results indicate that splenic B1 cells from autoimmune (NZB X NZW) F1 mice have a comparable responsiveness to the CD40‐mediated stimulation to that of B2 cells, which would be a potent regulatory mechanism involved in the spontaneous production of autoantibodies by B1 cells.
影响因子：
4.4
作者：
C. Mohan;Y. Shi;J. Laman;S. Datta
通讯作者：
C. Mohan;Y. Shi;J. Laman;S. Datta
影响因子：
4.4
作者：
Waldschmidt,TJ;Kroese,FG;Tygrett,LT;Conrad,DH;Lynch,RG
通讯作者：
Lynch,RG
原代 B 细胞对细菌脂多糖 O/核心区域的反应仅限于 Ly-1 谱系。
DOI：
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发表时间：
1991
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Su,SD;Ward,MM;Apicella,MA;Ward,RE
通讯作者：
Ward,RE