ACTB, CDKN1B, GAPDH, GRB2, RHOA and SDCBP Were Identified as Reference Genes in Neuroendocrine Lung Cancer via the nCounter Technology.
ACTB, CDKN1B, GAPDH, GRB2, RHOA and SDCBP Were Identified as Reference Genes in Neuroendocrine Lung Cancer via the nCounter Technology.
复制标题
DOI：
10.1371/journal.pone.0165181
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Mairinger FD
中科院分区：
文献类型：
--
作者：
Walter RF;Werner R;Vollbrecht C;Hager T;Flom E;Christoph DC;Schmeller J;Schmid KW;Wohlschlaeger J;Mairinger FD
神经内分泌肺癌（NELC）占所有肺癌病例的25%，并且大的患者群体仅以福尔马林固定、石蜡包埋（FFPE）组织存在。FFPE作为分子生物学分析的来源存在争议，NELC的参考基因建立得很差。使用数字nCounter技术（NanoString）对43份代表性FFPE标本进行mRNA表达分析。基于最近的文献，总共91个mRNA靶点被研究作为潜在的肿瘤标志物或参考基因。采用geNorm、Normalization算法和相关系数等方法确定最稳定的参考基因。使用R编程环境（版本3.1.1）进行统计分析，RNA完整性（RIN）范围为1.8 - 2.6，浓度范围为34 - 2，109 ng/μl。然而，nCounter技术为所有受试样本提供了可评价的结果。ACTB、CDKN 1B、GAPDH、GRB 2、RHOA和SDCBP被鉴定为具有高稳定性（M-）值的恒定表达基因。FFPE衍生的mRNA适用于通过nCounter技术进行分子生物学研究，尽管它是高度降解的。ACTB、CDKN 1B、GAPDH、GRB 2、RHOA和SDCBP是肺神经内分泌肿瘤中的有效参考基因。
Neuroendocrine lung cancer (NELC) represents 25% of all lung cancer cases and large patient collectives exist as formalin-fixed, paraffin-embedded (FFPE) tissue only. FFPE is controversially discussed as source for molecular biological analyses and reference genes for NELC are poorly establishes. Forty-three representative FFPE-specimens were used for mRNA expression analysis using the digital nCounter technology (NanoString). Based on recent literature, a total of 91 mRNA targets were investigated as potential tumor markers or reference genes. The geNorm, NormFinder algorithms and coefficient of correlation were used to identify the most stable reference genes. Statistical analysis was performed by using the R programming environment (version 3.1.1) RNA integrity (RIN) ranged from 1.8 to 2.6 and concentrations from 34 to 2,109 ng/μl. However, the nCounter technology gave evaluable results for all samples tested. ACTB, CDKN1B, GAPDH, GRB2, RHOA and SDCBP were identified as constantly expressed genes with high stability (M-)values according to geNorm, NormFinder and coefficients of correlation. FFPE-derived mRNA is suitable for molecular biological investigations via the nCounter technology, although it is highly degraded. ACTB, CDKN1B, GAPDH, GRB2, RHOA and SDCBP are potent reference genes in neuroendocrine tumors of the lung.
登录
查看更多内容
影响因子：
3.9
作者：
Mairinger FD;Walter RF;Theegarten D;Hager T;Vollbrecht C;Christoph DC;Worm K;Ting S;Werner R;Stamatis G;Mairinger T;Baba H;Zarogoulidis K;Huang H;Li Q;Tsakiridis K;Zarogoulidis P;Schmid KW;Wohlschlaeger J
通讯作者：
Wohlschlaeger J
影响因子：
8.8
作者：
Vollbrecht C;Werner R;Walter RF;Christoph DC;Heukamp LC;Peifer M;Hirsch B;Burbat L;Mairinger T;Schmid KW;Wohlschlaeger J;Mairinger FD
通讯作者：
Mairinger FD
影响因子：
2.2
作者：
Beaume, Marie;Hernandez, David;Francois, Patrice
通讯作者：
Francois, Patrice
影响因子：
2.8
作者：
Walter, Robert Fred Henry;Mairinger, Fabian Dominik;Hager, Thomas
通讯作者：
Hager, Thomas
血管生成的激活在肺类癌和神经内分泌癌之间存在很大差异，并且对于类癌肿瘤的发生至关重要。
影响因子：
3.9
作者：
Mairinger FD;Walter RF;Werner R;Christoph DC;Ting S;Vollbrecht C;Zarogoulidis K;Huang H;Li Q;Schmid KW;Wohlschlaeger J;Zarogoulidis P
通讯作者：
Zarogoulidis P