The DinB superfamily includes novel mycothiol, bacillithiol, and glutathione S-transferases.
The DinB superfamily includes novel mycothiol, bacillithiol, and glutathione S-transferases.
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DOI：
10.1021/bi201460j
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发表时间：
2011-12-13
期刊：
影响因子：
2.9
通讯作者：
Fahey, Robert C.
中科院分区：
文献类型：
--
作者：
Newton, Gerald L.;Leung, Stephan S.;Wakabayashi, Judy I.;Rawat, Mamta;Fahey, Robert C.
谷胱甘肽S-转移酶的超家族已经成为广泛研究的主题，但是放线菌产生真菌硫醇（MSH）代替谷胱甘肽，并且没有鉴定出真菌硫醇S-转移酶（MST）。使用真菌硫醇和一氯二亚胺作为底物，在耻垢分枝杆菌提取物中检测到MST活性，并进行充分纯化，以将DinB超家族DUF 664家族成员MSMEG_0887鉴定为MST。M.通过克隆证实了结核分枝杆菌和同源结核分枝杆菌（Rv 0443）的酶，并且发现表达的蛋白质与MSH有活性，但与杆菌硫醇（BSH）或谷胱甘肽（GSH）无活性。枯草芽孢杆菌YfiT是DinB超家族的另一个成员，但这种细菌产生BSH。当用BSH而不是用MSH或GSH测定时，显示YfiT蛋白对一氯二亚胺具有S-转移酶活性。粪肠球菌EF_3021与MSMEG_0887具有一定的同源性，但该微生物产生GSH，但不产生MSH或BSH。克隆并表达的EF_0321对一氯二亚胺和GSH有活性，但对MSH和BSH无活性。MDMPI_2是DinB超家族的另一个成员，并且先前已显示具有真菌硫醇依赖性马来酰丙酮酸异构酶活性。DinB超家族的八个家族中的三个家族包括显示催化硫醇依赖性代谢或解毒活性的蛋白质。由于超过三分之二的序列分配给DinB超家族的成员，似乎这样的活动是主导的DinB超家族。
The superfamily of glutathione S-transferases has been the subject of extensive study but Actinobacteria produce mycothiol (MSH) in place of glutathione and no mycothiol S-transferase (MST) has been identified. Using mycothiol and monochlorobimane as substrates a MST activity was detected in extracts of Mycobacterium smegmatis and purified sufficiently to allow identification of MSMEG_0887, a member the DUF664 family of the DinB superfamily, as the MST. The identity of the M. smegmatis and homologous Mycobacterium tuberculosis (Rv0443) enzymes was confirmed by cloning and the expressed proteins were found to be active with MSH but not bacillithiol (BSH) or glutathione (GSH). Bacillus subtilis YfiT is another member of the DinB superfamily but this bacterium produces BSH. The YfiT protein was shown to have S-transferase activity with monochlorobimane when assayed with BSH but not with MSH or GSH. Enterococcus faecalis EF_3021 shares some homology with MSMEG_0887 but this organism produces GSH but not MSH or BSH. Cloned and expressed EF_0321 was active with monochlorobimane and GSH but not with MSH or BSH. MDMPI_2 is another member of the DinB superfamily and has been previously shown to have mycothiol-dependent maleylpyruvate isomerase activity. Three of the eight families of the DinB superfamily include proteins shown to catalyze thiol-dependent metabolic or detoxification activities. Since more than two-thirds of the sequences assigned to the DinB superfamily are members of these families it seems likely that such activity is dominant in the DinB superfamily.
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影响因子：
2.3
作者：
Hori M;Yonekura S;Nohmi T;Gruz P;Sugiyama H;Yonei S;Zhang-Akiyama QM
通讯作者：
Zhang-Akiyama QM
影响因子：
4.1
作者：
CLARK, AG;SMITH, JN;SPEIR, TW
通讯作者：
SPEIR, TW
影响因子：
4.5
作者：
Galhano, Victor;Gomes-Laranjo, Jose;Peixoto, Francisco
通讯作者：
Peixoto, Francisco
影响因子：
4.8
作者：
Feng, J;Che, YS;Liu, SJ
通讯作者：
Liu, SJ
DOI：
10.1016/0360-3016(89)90307-6
发表时间：
1989-05-01
影响因子：
7
作者：
COOK, JA;PASS, HI;MITCHELL, JB
通讯作者：
MITCHELL, JB