Comparative analysis of chromatin landscape in regulatory regions of human housekeeping and tissue specific genes.
Comparative analysis of chromatin landscape in regulatory regions of human housekeeping and tissue specific genes.
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DOI：
10.1186/1471-2105-6-126
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发表时间：
2005-05-26
影响因子：
3
通讯作者：
Brahmachari SK
中科院分区：
文献类型：
--
作者：
Ganapathi M;Srivastava P;Das Sutar SK;Kumar K;Dasgupta D;Pal Singh G;Brahmachari V;Brahmachari SK
涉及基因启动子区的染色质组装和重塑的全局调控机制涉及真核生物的转录控制，特别是对于受到空间和时间调控的基因。利用全局调控机制控制基因表达的潜力可能取决于基因内部和周围染色质的结构。计算机模拟分析可以对这方面产生重要的见解，促进两个或更多个类别的基因的比较，这些基因包括每组内的大量基因。在本研究中，我们进行了比较分析的染色质特征的支架/基质附着区，核小体形成的潜力和重复序列的发生，在上游调控区的管家和组织特异性基因。我们的数据显示，与管家基因相比，在组织特异性基因的5 '区域中，推定的支架/基质附着区域更丰富，并且核小体形成潜力更高。两组基因之间染色质特征的差异表明染色质组织参与基因表达的控制。通过染色质组织介导的全局调节机制的存在可以减少调用基因特异性调节剂以维持基因表达的活性/沉默状态的负担。这可以部分解释人类基因组中估计的基因数量较低。
Global regulatory mechanisms involving chromatin assembly and remodelling in the promoter regions of genes is implicated in eukaryotic transcription control especially for genes subjected to spatial and temporal regulation. The potential to utilise global regulatory mechanisms for controlling gene expression might depend upon the architecture of the chromatin in and around the gene. In-silico analysis can yield important insights into this aspect, facilitating comparison of two or more classes of genes comprising of a large number of genes within each group. In the present study, we carried out a comparative analysis of chromatin characteristics in terms of the scaffold/matrix attachment regions, nucleosome formation potential and the occurrence of repetitive sequences, in the upstream regulatory regions of housekeeping and tissue specific genes. Our data show that putative scaffold/matrix attachment regions are more abundant and nucleosome formation potential is higher in the 5' regions of tissue specific genes as compared to the housekeeping genes. The differences in the chromatin features between the two groups of genes indicate the involvement of chromatin organisation in the control of gene expression. The presence of global regulatory mechanisms mediated through chromatin organisation can decrease the burden of invoking gene specific regulators for maintenance of the active/silenced state of gene expression. This could partially explain the lower number of genes estimated in the human genome.
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影响因子：
5.8
作者：
Grover, D;Mukerji, M;Brahmachari, SK
通讯作者：
Brahmachari, SK
影响因子：
9.5
作者：
Rogozin, I B;Glazko, G V;Glazkov, M V
通讯作者：
Glazkov, M V
影响因子：
14.9
作者：
Singh, GB;Kramer, JA;Krawetz, SA
通讯作者：
Krawetz, SA
影响因子：
14.9
作者：
BONIFER, C;YANNOUTSOS, N;SIPPEL, AE
通讯作者：
SIPPEL, AE
影响因子：
64.5
作者：
Gilbert, N;Boyle, S;Bickmore, WA
通讯作者：
Bickmore, WA