Ethanol‐induced changes in hepatic free radical defense mechanisms and fatty‐acid composition in the miniature pig
Ethanol‐induced changes in hepatic free radical defense mechanisms and fatty‐acid composition in the miniature pig
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乙醇诱导小型猪肝脏自由基防御机制和脂肪酸组成的变化
DOI：
10.1002/hep.1840130627
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发表时间：
1991
期刊：
影响因子：
13.5
通讯作者：
C. Keen
中科院分区：
文献类型：
--
作者：
S. Zidenberg;K. Olin;J. Villanueva;A. Tang;S. Phinney;C. Halsted;C. Keen
据报道，在小型猪中，乙醇消耗会引起肝脏抗氧化防御能力的改变，这可能导致过氧化损伤的风险增加。然而，乙醇也可能引起膜脂肪酸组成的变化，从而降低过氧化损伤的风险。这项研究检查了小型猪肝脏中的脂质过氧化、抗氧化防御和脂肪酸组成，这些小型猪在 20 个月内以含有 12% 热量的脂肪的饮食饲喂乙醇。饲喂12个月和20个月后，与对照组相比，乙醇饲喂的猪具有较高的肝锰超氧化物歧化酶活性、较低的肝铜浓度以及较低的肝铜锌超氧化物歧化酶和谷胱甘肽过氧化物酶活性。通过硫代巴比妥酸反应物质测定评估，乙醇喂养猪的肝匀浆、线粒体和微粒体部分中的脂质过氧化水平低于对照组。乙醇饲喂猪的肝脏匀浆（饲喂 12 个月后）和微粒体部分（饲喂 20 个月后）中高度不饱和脂肪酸占总脂肪酸的百分比低于对照组，从而导致过氧化指数较低。通过组织学方法评估，乙醇喂养的猪的肝损伤极小或没有。我们认为，微粒体对脂质过氧化的相对抵抗力是由于乙醇饲喂猪的过氧化指数较低，并且可能部分解释了饲喂乙醇 20 个月后没有显着组织病理学发现的原因。 （肝病学 1991；13：1185–1192。）
In the miniature pig, ethanol consumption has been reported to induce alterations in hepatic antioxidant defense capacity, which could result in increased risk of peroxidative damage. However, ethanol may also induce changes in membrane fatty acid composition, which could reduce the risk of peroxidative damage. This study examined lipid peroxidation, antioxidant defense and fatty acid composition in livers from miniature pigs fed ethanol in diets containing 12% of their calories as fat for 20 mo. After 12 and 20 mo of feeding, ethanol‐fed pigs had higher hepatic manganese–superoxide dismutase activity, lower hepatic copper concentrations and low hepatic copperzinc–superoxide dismutase and glutathione peroxidase activities compared with controls. Lipid peroxidation as assessed by thiobarbituric acid reacting substance assay was lower in liver homogenate and mitochondrial and microsomal fractions from ethanol‐fed pigs than in controls. The percentage contribution of highly unsaturated fatty acids to total fatty acids in liver homogenates (after 12 mo of feeding) and microsome fractions (after 20 mo of feeding) was lower in the ethanol‐fed pigs than in the controls, resulting in a lower peroxidizability index. Ethanol‐fed pigs had minimal or no hepatic damage as assessed by histological methods. We suggest that the relative resistance of microsomes to lipid peroxidation is due to the lower peroxidizability index in the ethanol‐fed pigs and may account in part for the absence of significant histopathological findings after 20 mo of ethanol feeding. (HEPATOLOGY 1991;13:1185–1192.)
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酒精猪的叶酸吸收：体内肠道灌注研究。
DOI：
10.1093/ajcn/50.6.1429
发表时间：
1989
期刊：
The American journal of clinical nutrition
影响因子：
--
作者：
Reisenauer,AM;Buffington,CA;Villanueva,JA;Halsted,CH
通讯作者：
Halsted,CH
慢性酒精摄入对小型猪自由基防御的影响。
DOI：
10.1093/jn/120.2.213
发表时间：
1990
期刊：
The Journal of nutrition
影响因子：
--
作者：
Zidenberg-Cherr,S;Halsted,CH;Olin,KL;Reisenauer,AM;Keen,CL
通讯作者：
Keen,CL
影响因子：
7.4
作者：
FRAGA, CG;LEIBOVITZ, BE;TAPPEL, AL
通讯作者：
TAPPEL, AL
乙醇诱导的脂质过氧化：长期饮酒会增强，蛋氨酸会减弱。
DOI：
--
发表时间：
1981
期刊：
The Journal of laboratory and clinical medicine
影响因子：
--
作者：
Shaw,S;Jayatilleke,E;Ross,WA;Gordon,ER;Leiber,CS
通讯作者：
Leiber,CS