Short-term mechanical unloading with left ventricular assist devices after acute myocardial infarction conserves calcium cycling and improves heart function.
Short-term mechanical unloading with left ventricular assist devices after acute myocardial infarction conserves calcium cycling and improves heart function.
复制标题
急性心肌梗塞后使用左心室辅助装置进行短期机械减负荷可保存钙循环并改善心脏功能
DOI：
10.1016/j.jcin.2012.12.122
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发表时间：
2013-04
影响因子：
11.3
通讯作者：
Griffith, Bartley P.
中科院分区：
文献类型：
--
作者：
Wei, Xufeng;Li, Tieluo;Hagen, Brian;Zhang, Pei;Sanchez, Pablo G.;Williams, Katrina;Li, Shuying;Bianchi, Giacomo;Son, Ho Sung;Wu, Changfu;DeFilippi, Christopher;Xu, Kai;Lederer, William J.;Wu, Zhongjun J.;Griffith, Bartley P.
关键词：
本研究旨在证明急性心肌梗死（MI）后使用左心室（LV）辅助装置（LVAD）进行短期心脏卸载可以保护钙循环并改善心脏功能。继发于MI的心力衰竭仍然是发病率和死亡率的主要来源。钙循环的改变与衰竭心脏的心功能障碍有关。成年Dorsett杂种羊发生急性MI，并使用轴流式LVAD（Impella 5.0）机械卸载2周（n = 6）。6只MI绵羊和4只假手术绵羊用作对照。所有动物在MI后随访12周。局部应变在LV测量声显微镜。主要的钙处理蛋白（CHP），包括肌浆网/内质网钙ATP酶-2 α（SERCA-2α）、Na+-Ca 2+交换体-1和受磷蛋白（phospholamban），以及Ca 2 +-ATP酶活性。采用膜片钳技术检测单个心肌细胞的钙循环。电镜观察相关超微结构。与MI组相比，LVAD去负荷减轻了左室扩张，改善了整体心功能和局部收缩力。局部心肌应变（牵张）在卸载期间最小化，甚至在12周时与MI组相比有所减弱。MI组邻近非梗死区钙循环明显受损，而LVAD卸载组CHP表达正常，Ca 2 +-ATP酶活性保留。无负荷组的电生理钙循环也得到了保护，超微结构损伤得到了改善。短期LVAD去负荷可以保护心肌梗死后钙循环，改善心功能。
This study sought to demonstrate that short-term cardiac unloading with a left ventricular (LV) assist device (LVAD) after acute myocardial infarction (MI) can conserve calcium cycling and improve heart function. Heart failure secondary to MI remains a major source of morbidity and mortality. Alterations in calcium cycling are linked to cardiac dysfunction in the failing heart. Adult Dorsett hybrid sheep underwent acute MI and were mechanically unloaded with an axial-flow LVAD (Impella 5.0) for 2 weeks (n = 6). Six sheep with MI only and 4 sham sheep were used as controls. All animals were followed for 12 weeks post-MI. Regional strains in the LV were measured by sonomicrometry. Major calcium-handling proteins (CHPs), including sarco-/endoplasmic reticulum calcium ATPase-2α (SERCA-2α), Na+-Ca2+ exchanger-1, and phospholamban, and Ca2+-ATPase activity were investigated. The electrophysiological calcium cycling in single isolated cardiomyocytes was measured with the patch-clamp technique. The related ultrastructures were studied with electron microscopy. LVAD unloading alleviated LV dilation and improved global cardiac function and regional contractility compared with the MI group. The regional myocardial strain (stretch) was minimized during the unloading period and even attenuated compared with the MI group at 12 weeks. Impaired calcium cycling was evident in the adjacent noninfarcted zone in the MI group, whereas CHP expression was normalized and Ca2+-ATPase activity was preserved in the LVAD unloading group. The electrophysiological calcium cycling was also conserved, and the ultrastructural damage was ameliorated in the unloaded animals. Short-term LVAD unloading may conserve calcium cycling and improve heart function during the post-infarct period.
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影响因子：
37.8
作者：
Lompré AM;Hajjar RJ;Harding SE;Kranias EG;Lohse MJ;Marks AR
通讯作者：
Marks AR
影响因子：
158.5
作者：
Rose, EA;Gelijns, AC;Meier, P
通讯作者：
Meier, P
DOI：
10.1016/j.ddmec.2007.12.006
发表时间：
2007-01-01
期刊：
Drug discovery today. Disease mechanisms
影响因子：
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作者：
French, Brent A;Kramer, Christopher M
通讯作者：
Kramer, Christopher M
影响因子：
158.5
作者：
Birks, Emma J.;Tansley, Patrick D.;Yacoub, Magdi H.
通讯作者：
Yacoub, Magdi H.
DOI：
10.1016/s0735-1097(02)02121-6
发表时间：
2002-09-18
影响因子：
24
作者：
Jackson, BM;Gorman, JH;Gorman, RC
通讯作者：
Gorman, RC