Evaluation of PCR cycle threshold values by patient population with the quidel lyra SARS-CoV-2 assay.
Evaluation of PCR cycle threshold values by patient population with the quidel lyra SARS-CoV-2 assay.
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DOI：
10.1016/j.diagmicrobio.2021.115387
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发表时间：
2021-10
影响因子：
2.9
通讯作者：
Parikh, Bijal A.
中科院分区：
文献类型：
--
作者：
Potter, Robert F.;Abro, Brooj;Eby, Charles S.;Burnham, Carey-Ann D.;Anderson, Neil W.;Parikh, Bijal A.
Lyra SARS-CoV-2检测是在2020年3月中旬至4月下旬最初的COVID-19激增期间在密苏里州圣路易斯的巴恩斯-犹太医疗保健系统进行的分子检测的主要方法。我们对这36天内1043例Lyra阳性SARS-CoV-2结果进行了回顾性分析，以调查周期阈值（CT）值与患者特征之间的关系。使用EasyMag或KingFisher自动提取系统从NP或OP棉签中提取总RNA，并分别使用RotorGene Q （Qiagen）或Applied Biosystems 7500 Fast Dx热循环仪进行定量。值得注意的是，与EasyMag/RotorGene （EM/RGQ）平台相比，我们发现在KingFisher-ABI 7500 fastDX （KF/ABI）系统上测试的样品中位CT值明显更低。由于我们的77.5%的测试是在EM/RGQ管道上进行的，我们随后对这些值进行了额外的分析，发现门诊护理环境中的C T值与急诊科或住院患者获得的样本相比，C T值显着降低。这些集体发现表明，在不同的患者群体中，病毒载量存在差异。
The Lyra SARS-CoV-2 assay was the primary method for molecular testing performed at Barnes-Jewish Healthcare System in St. Louis, Missouri during the initial COVID-19 surge from mid-March to late-April 2020. We performed a retrospective analysis of 1,043 positive Lyra SARS-CoV-2 results during these 36 days to investigate associations between cycle threshold (CT) value and patient characteristics. Total RNA were extracted from NP or OP swabs using either the EasyMag or KingFisher automated extraction systems and quantified with RotorGene Q (Qiagen) or Applied Biosystems 7500 Fast Dx thermocyclers respectively. Notably, we found lower a significant median lower CT for samples tested on the KingFisher-ABI 7500 fastDX (KF/ABI) system compared to the EasyMag/RotorGene (EM/RGQ) platform. Since 77.5% of our tests were ran on the EM/RGQ pipeline we then perform additional analysis on these values and found that C T values in outpatient care settings compared to samples obtained in the emergency department or inpatient had significantly lower C T values. These collective findings suggests a difference in viral load amongst various patient populations.
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