Nickel-resistance determinants in Acidiphilium sp. PM identified by genome-wide functional screening.
Nickel-resistance determinants in Acidiphilium sp. PM identified by genome-wide functional screening.
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DOI：
10.1371/journal.pone.0095041
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Gonzalez-Pastor JE
中科院分区：
文献类型：
--
作者：
San Martin-Uriz P;Mirete S;Alcolea PJ;Gomez MJ;Amils R;Gonzalez-Pastor JE
嗜酸菌Acidiphilium spp.是酸性、富含金属的环境中的显眼居住者。事实上，它们是最耐金属的生物之一；然而，人们对该属耐金属背后的机制知之甚少。嗜酸菌Acidiphilium sp.PM是与力拓隔离的环境，力拓是一条位于西班牙西南部的酸性、富含金属的河流。对其金属电阻的表征表明，它具有显著的耐高镍浓度的能力。本文报道了嗜酸菌基因组文库的筛选。PM以确定与镍抗性相关的基因。这一方法揭示了七个不同的基因与大肠杆菌对镍的抗性有关，其中两个基因形成了编码ATP依赖的蛋白酶HslVU(ClpQY)的操纵子。这种酶被发现能增强大肠杆菌对镍和钴的抗性，这一功能以前没有报道过。其他抗镍决定因素包括与内毒素生物合成和分支氨基酸合成有关的基因。筛选中回收的基因的分子功能多样性表明，嗜酸杆菌对镍的抗性。PM可能依赖于不同的分子机制。
Acidiphilium spp. are conspicuous dwellers of acidic, metal-rich environments. Indeed, they are among the most metal-resistant organisms; yet little is known about the mechanisms behind the metal tolerance in this genus. Acidiphilium sp. PM is an environmental isolate from Rio Tinto, an acidic, metal-laden river located in southwestern Spain. The characterization of its metal resistance revealed a remarkable ability to tolerate high Ni concentrations. Here we report the screening of a genomic library of Acidiphilium sp. PM to identify genes involved in Ni resistance. This approach revealed seven different genes conferring Ni resistance to E. coli, two of which form an operon encoding the ATP-dependent protease HslVU (ClpQY). This protease was found to enhance resistance to both Ni and Co in E. coli, a function not previously reported. Other Ni-resistance determinants include genes involved in lipopolysaccharide biosynthesis and the synthesis of branched amino acids. The diversity of molecular functions of the genes recovered in the screening suggests that Ni resistance in Acidiphilium sp. PM probably relies on different molecular mechanisms.
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