Metagenomic identification of novel viruses of maize and teosinte in North America.
Metagenomic identification of novel viruses of maize and teosinte in North America.
复制标题
DOI：
10.1186/s12864-022-09001-w
复制
发表时间：
2022-11-23
期刊：
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
--
作者：
众所周知，玉米感染病毒每年在世界各地造成重大的农业产量损失。在疾病爆发之前确定已知或新的致病因子对于通过未来的作物保护工作来维护粮食安全至关重要。为了实现这一目标，利用高通量测序（HTS）的大规模宏基因组方法被用于鉴定可能导致北美谷物物种（特别是玉米）产量损失的新型病毒。在这里，我们提出了四种由HTS发现的新型病毒，并分别通过Sanger测序进行了验证。其中三种病毒是RNA病毒，属于Betaflexiviridae或Tombusviridae科。此外，还发现了一种属于双病毒科的新型DNA病毒，这是在北美玉米中首次发现的Mastrevirus。诸如此类的作物及其相关物种的宏基因组研究可能有助于识别和监测作物的已知和新型病毒病原体。监测相关物种可能有助于识别由于昆虫媒介和病毒宿主范围重叠而能够感染作物的病毒，从而保护粮食安全。在线版本包含补充材料，可在10.1186/s12864-022-09001-w获得。
Maize-infecting viruses are known to inflict significant agronomic yield loss throughout the world annually. Identification of known or novel causal agents of disease prior to outbreak is imperative to preserve food security via future crop protection efforts. Toward this goal, a large-scale metagenomic approach utilizing high throughput sequencing (HTS) was employed to identify novel viruses with the potential to contribute to yield loss of graminaceous species, particularly maize, in North America. Here we present four novel viruses discovered by HTS and individually validated by Sanger sequencing. Three of these viruses are RNA viruses belonging to either the Betaflexiviridae or Tombusviridae families. Additionally, a novel DNA virus belonging to the Geminiviridae family was discovered, the first Mastrevirus identified in North American maize. Metagenomic studies of crop and crop-related species such as this may be useful for the identification and surveillance of known and novel viral pathogens of crops. Monitoring related species may prove useful in identifying viruses capable of infecting crops due to overlapping insect vectors and viral host-range to protect food security. The online version contains supplementary material available at 10.1186/s12864-022-09001-w.
登录
查看更多内容
DOI：
10.3390/plants10020233
发表时间：
2021-01-25
期刊：
Plants (Basel, Switzerland)
影响因子：
--
作者：
Jones RAC
通讯作者：
Jones RAC
影响因子：
3.7
作者：
Téllez-Sosa J;Rodríguez MH;Gómez-Barreto RE;Valdovinos-Torres H;Hidalgo AC;Cruz-Hervert P;Luna RS;Carrillo-Valenzo E;Ramos C;García-García L;Martínez-Barnetche J
通讯作者：
Martínez-Barnetche J
影响因子：
2.7
作者：
Adams, MJ;Antoniw, JF;Fauquet, CM
通讯作者：
Fauquet, CM
影响因子：
5.4
作者：
Arguello-Astorga, G;Lopez-Ochoa, L;Hanley-Bowdoin, L
通讯作者：
Hanley-Bowdoin, L
影响因子：
14.9
作者：
Boratyn GM;Camacho C;Cooper PS;Coulouris G;Fong A;Ma N;Madden TL;Matten WT;McGinnis SD;Merezhuk Y;Raytselis Y;Sayers EW;Tao T;Ye J;Zaretskaya I
通讯作者：
Zaretskaya I