The small RNA SgrS controls sugar-phosphate accumulation by regulating multiple PTS genes.
The small RNA SgrS controls sugar-phosphate accumulation by regulating multiple PTS genes.
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DOI：
10.1093/nar/gkq1219
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发表时间：
2011-05
影响因子：
14.9
通讯作者：
Vanderpool CK
中科院分区：
文献类型：
--
作者：
Rice JB;Vanderpool CK
许多细菌小RNA（sRNA）通过控制多个基因的表达而作为胁迫反应的全局调节剂。sRNA SgrS响应于葡萄糖-磷酸盐应激而表达，葡萄糖-磷酸盐应激是与糖酵解通量的破坏和糖-磷酸盐的积累相关的病症。SgrS已显示刺激编码主要葡萄糖转运蛋白的ptsG mRNA的降解。这项研究表明，SgrS调节编码甘露糖和二级葡萄糖转运蛋白manXYZ的基因。在存在和不存在SgrS的情况下对manXYZ mRNA稳定性和翻译的分析表明，在应激条件下和当SgrS异位表达时，manXYZ受SgrS调节。体外足迹法和体内突变分析表明，SgrS与manX编码序列内的manXYZ碱基配对，以防止manX翻译。manX的调节不需要RNase E降解体复合物，这表明调节的主要机制是翻译。当暴露于葡萄糖类似物α-甲基葡糖苷或2-脱氧葡萄糖时，manXYZ+的大肠杆菌ptsG突变株经历应激。一个ptsG manXYZ双突变体是耐应力，表明PTS转运蛋白编码的两个SgrS目标参与采取的底物，造成压力。
A number of bacterial small RNAs (sRNAs) act as global regulators of stress responses by controlling expression of multiple genes. The sRNA SgrS is expressed in response to glucose–phosphate stress, a condition associated with disruption of glycolytic flux and accumulation of sugar–phosphates. SgrS has been shown to stimulate degradation of the ptsG mRNA, encoding the major glucose transporter. This study demonstrates that SgrS regulates the genes encoding the mannose and secondary glucose transporter, manXYZ. Analysis of manXYZ mRNA stability and translation in the presence and absence of SgrS indicate that manXYZ is regulated by SgrS under stress conditions and when SgrS is ectopically expressed. In vitro footprinting and in vivo mutational analyses showed that SgrS base pairs with manXYZ within the manX coding sequence to prevent manX translation. Regulation of manX did not require the RNase E degradosome complex, suggesting that the primary mechanism of regulation is translational. An Escherichia coli ptsG mutant strain that is manXYZ+ experiences stress when exposed to the glucose analogs α-methyl glucoside or 2-deoxyglucose. A ptsG manXYZ double mutant is resistant to the stress, indicating that PTS transporters encoded by both SgrS targets are involved in taking up substrates that cause stress.
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