Identification of an epigenetic biomarker panel with high sensitivity and specificity for colorectal cancer and adenomas.
Identification of an epigenetic biomarker panel with high sensitivity and specificity for colorectal cancer and adenomas.
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鉴定对结直肠癌和腺瘤具有高灵敏度和特异性的表观遗传生物标记物面板。
DOI：
10.1186/1476-4598-10-85
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发表时间：
2011-07-21
期刊：
影响因子：
37.3
通讯作者：
Lothe RA
中科院分区：
文献类型：
--
作者：
Lind GE;Danielsen SA;Ahlquist T;Merok MA;Andresen K;Skotheim RI;Hektoen M;Rognum TO;Meling GI;Hoff G;Bretthauer M;Thiis-Evensen E;Nesbakken A;Lothe RA
人类粪便中上皮结直肠细胞中癌症特异性 DNA 甲基化模式的存在为结直肠癌及其前体腺瘤的简单、非侵入性筛查测试提供了前景。这项研究调查了一组用于检测结直肠癌和腺瘤的表观遗传标记。对来自结直肠癌、腺瘤和正常结肠粘膜的组织样本测试组中的候选生物标志物进行定量甲基化分析。所有发现均在独立的临床验证系列中得到验证。该研究总共纳入了 523 个人类样本。使用受试者工作特征（ROC）曲线分析来评估生物标志物组的性能。 CNRIP1、FBN1、INA、MAL、SNCA 和 SPG20 基因的启动子高甲基化在结直肠癌 (65-94%) 和腺瘤 (35-91%) 中很常见，而正常粘膜样本很少 (0-5%) 甲基化。六种标志物中至少两种阳性的组合敏感性对于结直肠癌样本为 94%，对于腺瘤样本为 93%，特异性为 98%。与正常粘膜相比，癌症的 ROC 曲线下面积为 0.984，腺瘤的 ROC 曲线下面积为 0.968。新型表观遗传标记物组对结直肠癌和腺瘤显示出非常高的敏感性和特异性。我们的研究结果表明该生物标志物组非常适合早期肿瘤检测。
The presence of cancer-specific DNA methylation patterns in epithelial colorectal cells in human feces provides the prospect of a simple, non-invasive screening test for colorectal cancer and its precursor, the adenoma. This study investigates a panel of epigenetic markers for the detection of colorectal cancer and adenomas. Candidate biomarkers were subjected to quantitative methylation analysis in test sets of tissue samples from colorectal cancers, adenomas, and normal colonic mucosa. All findings were verified in independent clinical validation series. A total of 523 human samples were included in the study. Receiver operating characteristic (ROC) curve analysis was used to evaluate the performance of the biomarker panel. Promoter hypermethylation of the genes CNRIP1, FBN1, INA, MAL, SNCA, and SPG20 was frequent in both colorectal cancers (65-94%) and adenomas (35-91%), whereas normal mucosa samples were rarely (0-5%) methylated. The combined sensitivity of at least two positives among the six markers was 94% for colorectal cancers and 93% for adenoma samples, with a specificity of 98%. The resulting areas under the ROC curve were 0.984 for cancers and 0.968 for adenomas versus normal mucosa. The novel epigenetic marker panel shows very high sensitivity and specificity for both colorectal cancers and adenomas. Our findings suggest this biomarker panel to be highly suitable for early tumor detection.
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影响因子：
3.1
作者：
Huang, Zhaohui;Li, Lihua;Wang, Jinfu
通讯作者：
Wang, Jinfu
影响因子：
--
作者：
Kleivi, K;Teixeira, MR;Lothe, RA
通讯作者：
Lothe, RA
影响因子：
10.3
作者：
Chen, WD;Han, ZJ;Markowitz, SD
通讯作者：
Markowitz, SD
影响因子：
12.3
作者：
Kilpinen, Sami;Autio, Reija;Ojala, Kalle;Iljin, Kristiina;Bucher, Elmar;Sara, Henri;Pisto, Tommi;Saarela, Matti;Skotheim, Rolf I.;Bjorkman, Mari;Mpindi, John-Patrick;Haapa-Paananen, Saija;Vainio, Paula;Edgren, Henrik;Wolf, Maija;Astola, Jaakko;Nees, Matthias;Hautaniemi, Sampsa;Kallioniemi, Olli
通讯作者：
Kallioniemi, Olli
影响因子：
7.4
作者：
Lind GE;Ahlquist T;Kolberg M;Berg M;Eknaes M;Alonso MA;Kallioniemi A;Meling GI;Skotheim RI;Rognum TO;Thiis-Evensen E;Lothe RA
通讯作者：
Lothe RA