Integrated multi-omic data and analyses reveal the pathways underlying key ornamental traits in carnation flowers.
Integrated multi-omic data and analyses reveal the pathways underlying key ornamental traits in carnation flowers.
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综合多组学数据和分析揭示了康乃馨花关键观赏性状的潜在途径
DOI：
10.1111/pbi.13801
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发表时间：
2022-06
影响因子：
13.8
通讯作者：
Fu, Xiaopeng
中科院分区：
文献类型：
--
作者：
Zhang, Xiaoni;Lin, Shengnan;Peng, Dan;Wu, Quanshu;Liao, Xuezhu;Xiang, Kunli;Wang, Zehao;Tembrock, Luke R.;Bendahmane, Mohammed;Bao, Manzhu;Wu, Zhiqiang;Fu, Xiaopeng
康乃馨（Dianthus caryophyllus）是世界上最受欢迎的观赏花卉之一。虽然有许多关于康乃馨的研究，但其花的颜色、香味和重瓣花形成的潜在机制仍然未知。本文采用综合多组学方法，阐明了康乃馨最重要的观赏特征背后的遗传和生化途径。首先，我们组装了D. caryophyllus（‘Scarlet Queen’）的高质量染色体规模基因组（636 Mb， N50为14.67 Mb）。接下来，使用不同类型的仪器从花的不同部位在多个发育阶段生成一系列代谢组学数据集，以评估色素和挥发性化合物积累的时空差异。最后，生成转录组数据，将基因组、生化和形态模式联系起来，提出了一套形成花瓣颜色、重瓣花和香味产生等观赏性状的途径。其中，转录因子bHLHs、MYBs和WRKY44同源物被认为在控制花瓣颜色模式中起重要作用，松柏醇乙酰转移酶和丁香酚合成酶等基因参与丁香酚的合成。本文提出的基因组学、转录组学和代谢组学综合数据集为理解康乃馨花发育和显色的潜在途径提供了重要基础，进而可用于选择育种和基因编辑，以开发新的康乃馨品种。
Carnation (Dianthus caryophyllus) is one of the most popular ornamental flowers in the world. Although numerous studies on carnations exist, the underlying mechanisms of flower color, fragrance, and the formation of double flowers remain unknown. Here, we employed an integrated multi‐omics approach to elucidate the genetic and biochemical pathways underlying the most important ornamental features of carnation flowers. First, we assembled a high‐quality chromosome‐scale genome (636 Mb with contig N50 as 14.67 Mb) of D. caryophyllus, the ‘Scarlet Queen’. Next, a series of metabolomic datasets was generated with a variety of instrumentation types from different parts of the flower at multiple stages of development to assess spatial and temporal differences in the accumulation of pigment and volatile compounds. Finally, transcriptomic data were generated to link genomic, biochemical, and morphological patterns to propose a set of pathways by which ornamental traits such as petal coloration, double flowers, and fragrance production are formed. Among them, the transcription factors bHLHs, MYBs, and a WRKY44 homolog are proposed to be important in controlling petal color patterning and genes such as coniferyl alcohol acetyltransferase and eugenol synthase are involved in the synthesis of eugenol. The integrated dataset of genomics, transcriptomics, and metabolomics presented herein provides an important foundation for understanding the underlying pathways of flower development and coloration, which in turn can be used for selective breeding and gene editing for the development of novel carnation cultivars.
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