LC-MS/MS quantitation of esophagus disease blood serum glycoproteins by enrichment with hydrazide chemistry and lectin affinity chromatography.
LC-MS/MS quantitation of esophagus disease blood serum glycoproteins by enrichment with hydrazide chemistry and lectin affinity chromatography.
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DOI：
10.1021/pr500570m
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发表时间：
2014-11-07
影响因子：
4.4
通讯作者：
Mechref, Yehia
中科院分区：
文献类型：
--
作者：
Song, Ehwang;Zhu, Rui;Hammond, Zane T.;Mechref, Yehia
糖基化的变化已被证明与许多癌症类型的发展/恶性程度具有深刻的相关性。目前，两种主要的富集技术已被广泛应用于糖蛋白组学，即基于凝集素亲和层析（LAC）和基于酰肼化学（HC）的富集。在这里，我们报告的LC-MS/MS定量分析的人血清糖蛋白和糖肽与食管疾病的LAC和HC为基础的富集。使用两种富集技术进行蛋白质糖基化的单独和互补的定性和定量数据分析。分别采用化学计量学和统计学评价、PCA图或ANOVA检验来确定和确认候选癌症相关糖蛋白/糖肽生物标志物。在139个中，在两种富集技术中观察到59个共同的糖蛋白（42%重叠）。这种重叠与以前发表的研究非常相似。显著变化的糖蛋白/糖肽的定量和评价在LAC和HC富集之间是互补的。LC-ESI-MS/MS分析表明，7种由LAC富集的糖蛋白和11种由HC富集的糖蛋白在无病和有病队列之间显示出显著不同的丰度。多反应监测定量结果显示，在疾病队列中，通过LAC富集获得的13个糖肽和通过HC富集获得的10个糖基化位点存在统计学差异。
Changes in glycosylation have been shown to have a profound correlation with development/malignancy in many cancer types. Currently, two major enrichment techniques have been widely applied in glycoproteomics, namely, lectin affinity chromatography (LAC)-based and hydrazide chemistry (HC)-based enrichments. Here we report the LC–MS/MS quantitative analyses of human blood serum glycoproteins and glycopeptides associated with esophageal diseases by LAC- and HC-based enrichment. The separate and complementary qualitative and quantitative data analyses of protein glycosylation were performed using both enrichment techniques. Chemometric and statistical evaluations, PCA plots, or ANOVA test, respectively, were employed to determine and confirm candidate cancer-associated glycoprotein/glycopeptide biomarkers. Out of 139, 59 common glycoproteins (42% overlap) were observed in both enrichment techniques. This overlap is very similar to previously published studies. The quantitation and evaluation of significantly changed glycoproteins/glycopeptides are complementary between LAC and HC enrichments. LC–ESI–MS/MS analyses indicated that 7 glycoproteins enriched by LAC and 11 glycoproteins enriched by HC showed significantly different abundances between disease-free and disease cohorts. Multiple reaction monitoring quantitation resulted in 13 glycopeptides by LAC enrichment and 10 glycosylation sites by HC enrichment to be statistically different among disease cohorts.
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影响因子：
3.4
作者：
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通讯作者：
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影响因子：
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DOI：
10.1016/j.jchromb.2006.07.067
发表时间：
2007-01-01
影响因子：
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作者：
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通讯作者：
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