Knockdown of a putative alanine aminotransferase gene affects amino acid content and flight capacity in the Colorado potato beetle Leptinotarsa decemlineata
Knockdown of a putative alanine aminotransferase gene affects amino acid content and flight capacity in the Colorado potato beetle Leptinotarsa decemlineata
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假定的丙氨酸转氨酶基因的敲除会影响科罗拉多马铃薯甲虫 Leptinotarsa decemlineata 的氨基酸含量和飞行能力
DOI：
10.1007/s00726-015-1978-1
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发表时间：
2015-04
期刊：
影响因子：
3.5
通讯作者：
Li Guo？Qing
中科院分区：
文献类型：
--
作者：
Fu Kai？Yun;Lü Feng？Gong;Guo Wen？Chao;Li Guo？Qing
Alanine aminotransferase (ALT) plays important physiological and biochemical roles in insect. In this study, a full-length Ldalt cDNA was cloned from Leptinotarsa decemlineata. It was ubiquitously expressed in the eggs, larvae, pupae and adults. In the adults, Ldalt mRNA was widely distributed in thorax muscles, fat body, midgut, foregut, hindgut, Malpighian tubules, ventral ganglion and epidermis, with the expression levels from the highest to the lowest. Two double-stranded RNAs (dsRNAs) (dsLdalt1 and dsLdalt2) targeting Ldalt were constructed and bacterially expressed. After adults fed on dsLdalt1- and dsLdalt2-immersed foliage for 3 day, Ldalt mRNA abundance was significantly decreased by 79.5 and 71.1 %, and ALT activities were significantly reduced by 64.5 and 67.6 %, respectively. Moreover, silencing Ldalt affected free amino acid contents. Lysine was decreased by 100.0 and 100.0 %, and arginine was reduced by 87.5 and 89.4 %, respectively, in the hemolymph from dsLdalt1- and dsLdalt2-ingested beetles, compared with control ones. In contrast, proline was increased by 88.7 and 96.4 %. Furthermore, ingestion of dsLdalt1 and dsLdalt2 significantly decreased flight speed, shortened flight duration time and flight distance. In addition, knocking down Ldalt significantly increased adult mortality. These data imply that LdALT plays important roles in amino acid metabolism and in flight in L. decemlineata.
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科罗拉多马铃薯甲虫 Leptinotarsa decemlineata 中假定的脯氨酸脱氢酶基因的分子克隆和表征
影响因子：
4
作者：
Ahmat Tursun;Yang Lu;Mu Li-Li;Li Guo-Qing
通讯作者：
Li Guo-Qing
影响因子：
3.6
作者：
Almeida AD;Wise HM;Hindley CJ;Slevin MK;Hartley RS;Philpott A
通讯作者：
Philpott A
DOI：
10.1111/j.1432-1033.1993.tb17953.x
发表时间：
1993-06-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
MEHTA, PK;HALE, TI;CHRISTEN, P
通讯作者：
CHRISTEN, P
DOI：
10.1007/978-1-4615-9221-1_5
发表时间：
1981
期刊：
--
影响因子：
--
作者：
E. Bursell
通讯作者：
E. Bursell
DOI：
10.1016/j.ics.2004.08.094
发表时间：
2004-12
期刊：
--
影响因子：
--
作者：
G. Gäde
通讯作者：
G. Gäde