Enterococcal Metabolite Cues Facilitate Interspecies Niche Modulation and Polymicrobial Infection.
Enterococcal Metabolite Cues Facilitate Interspecies Niche Modulation and Polymicrobial Infection.
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DOI：
10.1016/j.chom.2016.09.004
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发表时间：
2016-10-12
影响因子：
30.3
通讯作者：
Kline KA
中科院分区：
文献类型：
--
作者：
Keogh D;Tay WH;Ho YY;Dale JL;Chen S;Umashankar S;Williams RBH;Chen SL;Dunny GM;Kline KA
粪肠球菌经常与泌尿道、留置导管以及手术伤口部位的多微生物感染相关。在伤口感染中，粪肠球菌与大肠杆菌及其他病原体共存，但控制多微生物定植和发病机制的原理尚不明确。在感染过程中，细菌必须克服多种宿主防御机制，包括营养性铁限制，才能持续存在并引发疾病。在本研究中，我们探究了当铁的可利用性受到限制时，粪肠球菌对混合菌种感染的作用。我们发现，粪肠球菌通过输出L - 鸟氨酸，在体外和体内显著增强大肠杆菌生物膜的生长和存活。这种代谢信号促进大肠杆菌合成肠杆菌素铁载体，使大肠杆菌能在限制其生长的铁限制条件下生长和形成生物膜。因此，粪肠球菌通过提供促进生长的信号来调节其局部环境，使共同感染的生物体克服铁限制，从而促进多微生物感染。
Enterococcus faecalis is frequently associated with polymicrobial infections of the urinary tract, indwelling catheters, and surgical wound sites. E. faecalis co-exists with Escherichia coli and other pathogens in wound infections, but mechanisms that govern polymicrobial colonization and pathogenesis are poorly defined. During infection, bacteria must overcome multiple host defenses, including nutrient iron limitation, to persist and cause disease. In this study, we investigated the contribution of E. faecalis to mixed-species infection when iron availability is restricted. We show that E. faecalis significantly augments E. coli biofilm growth and survival in vitro and in vivo by exporting L-ornithine. This metabolic cue facilitates E. coli biosynthesis of the enterobactin siderophore, allowing E. coli growth and biofilm formation in iron-limiting conditions that would otherwise restrict its growth. Thus, E. faecalis modulates its local environment by contributing growth-promoting cues that allow co-infecting organisms to overcome iron limitation and promotes polymicrobial infections.
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影响因子：
14.8
作者：
Chaturvedi, Kaveri S.;Hung, Chia S.;Crowley, Jan R.;Stapleton, Ann E.;Henderson, Jeffrey P.
通讯作者：
Henderson, Jeffrey P.
影响因子：
3.7
作者：
Dalton T;Dowd SE;Wolcott RD;Sun Y;Watters C;Griswold JA;Rumbaugh KP
通讯作者：
Rumbaugh KP
影响因子：
6.7
作者：
Henderson, Jeffrey P.;Crowley, Jan R.;Pinkner, Jerome S.;Walker, Jennifer N.;Tsukayama, Pablo;Stamm, Walter E.;Hooton, Thomas M.;Hultgren, Scott J.
通讯作者：
Hultgren, Scott J.
DOI：
10.1038/nrmicro3432
发表时间：
2015-05
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Flores-Mireles AL;Walker JN;Caparon M;Hultgren SJ
通讯作者：
Hultgren SJ
DOI：
10.1073/pnas.0737682100
发表时间：
2003-04-01
影响因子：
11.1
作者：
Hantke, K;Nicholson, G;Winkelmann, G
通讯作者：
Winkelmann, G