Mouse and human SHPS-1: molecular cloning of cDNAs and chromosomal localization of genes.
Mouse and human SHPS-1: molecular cloning of cDNAs and chromosomal localization of genes.
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小鼠和人类 SHPS-1：cDNA 的分子克隆和基因的染色体定位。
DOI：
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
M. Kasuga
中科院分区：
文献类型：
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作者：
T. Yamao;T. Matozaki;K. Amano;Y. Matsuda;N. Takahashi;F. Ochi;Y. Fujioka;M. Kasuga
SHPS-1(SHP底物-1)是一种糖基化受体样蛋白，胞外区有3个免疫球蛋白样结构域，胞内区有4个YXX(L/V/I)基序，分别是潜在的酪氨酸磷酸化和SRC同源2(SH2)结构域结合部位。不同的有丝分裂原和细胞黏附诱导SHPS-1的酪氨酸磷酸化以及随后与SHP-2和SH2结构域蛋白酪氨酸磷酸酶的结合，提示SHPS-1在细胞对生长因子和细胞黏附的应答信号中发挥作用。现在已经分离出了编码SHPS-1的小鼠和人类的cDNA。所推导的大鼠、人和小鼠SHPS-1的氨基酸序列具有65%到81%的同源性。除了SH2结构域结合位点外，在SHPS-1的细胞质区域还检测到一个富含Pro的SH3结构域结合位点。Northern印迹分析表明，人SHPS-1基因在脑中含量最高，小鼠早在第7天就存在于胚胎中。荧光原位杂交将SHPS-1基因定位在人染色体20p13和小鼠2号染色体F3带上。此外，种间回交分析将小鼠SHPS-1基因座分别定位在微卫星标记D2Mit63和D2Mit19的远端和近端，分别位于突变部位(Cm)、致死性乳汁(Lm)和良好部位(WE)。
SHPS-1 (SHP substrate-1) is a glycosylated receptor-like protein with three immunoglobulin-like domains in its extracellular region and four YXX(L/V/I) motifs, potential tyrosine phosphorylation and SRC homology 2 (SH2) domain binding sites, in its cytoplasmic region. Various mitogens and cell adhesion induce tyrosine phosphorylation of SHPS-1 and its subsequent association with SHP-2, and SH2 domain-containing protein tyrosine phosphatase, suggesting that SHPS-1 plays a role in cell signaling in response to both growth factors and cell adhesion. The mouse and human cDNAs encoding SHPS-1 have now been isolated. The deduced amino acid sequences of rat, human, and mouse SHPS-1 show identities of 65 to 81%. In addition to the SH2 domain binding sites, a proline-rich putative SH3 domain binding site was detected in the cytoplasmic region of SHPS-1. Northern blot analysis revealed that human SHPS-1 mRNA is most abundant in brain and that the mouse mRNA is present in embryos as early as day 7. Fluorescence in situ hybridization localized the SHPS-1 gene to human chromosome 20p13 and the F3 band of mouse chromosome 2. Furthermore, interspecific backcross analysis placed the mouse SHPS-1 locus 5.0 centimorgans distal and 1.4 centimorgans proximal to the microsatellite markers D2Mit63 and D2Mit19, respectively, in a region associated with the mutations coloboma (Cm), lethal milk (lm), and well-haarig (we).
胰岛素受体底物 1 与含有 SH2 的磷酸酪氨酸磷酸酶 Syp 结合。
DOI：
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发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kuhné,MR;Pawson,T;Lienhard,GE;Feng,GS
通讯作者：
Feng,GS
Syp (SH-PTP2) 是生长因子刺激的有丝分裂信号转导的正介体。
DOI：
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发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Xiao,S;Rose,DW;Sasaoka,T;Maegawa,H;BurkeJr,TR;Roller,PP;Shoelson,SE;Olefsky,JM
通讯作者：
Olefsky,JM
DOI：
10.1073/pnas.90.15.6939
发表时间：
1993-08-01
影响因子：
11.1
作者：
KAZLAUSKAS, A;FENG, GS;VALIUS, M
通讯作者：
VALIUS, M