Differential DNA Methylation and Gene Expression Between ALV-J-Positive and ALV-J-Negative Chickens.
Differential DNA Methylation and Gene Expression Between ALV-J-Positive and ALV-J-Negative Chickens.
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DOI：
10.3389/fvets.2021.659840
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发表时间：
2021
影响因子：
3.2
通讯作者：
Xie Q
中科院分区：
文献类型：
--
作者：
Yan Y;Zhang H;Gao S;Zhang H;Zhang X;Chen W;Lin W;Xie Q
背景资料：J亚群禽白血病病毒（Avian leukosis virus subgroup J，ALV-J）是一种致瘤病毒，给养禽业造成严重的经济损失，目前尚无有效的疫苗。DNA甲基化在多种生物学过程中起着至关重要的作用，越来越多的疾病已被证实与DNA甲基化改变有关。在本研究中，我们筛选ALV-J阳性和阴性鸡。随后，我们通过MeDIP-seq和RNA-seq生成并提供了ALV-J阳性和阴性鸡样本的全基因组基因表达和DNA甲基化谱;通过MeDIP-seq分析鉴定了8，304个差异甲基化区域（DMR）（p ≤ 0.005），通过RNA-seq分析鉴定了515个差异表达基因（p ≤ 0.05）。作为整合分析的结果，我们筛选了六个候选基因，以确定ALV-J阴性鸡具有差异甲基化的启动子区域。TGFB 2在肿瘤的发生和发展中起重要作用，提示TGFB 2可能是识别ALV-J感染的一个指标。
Background: Avian leukosis virus subgroup J (ALV-J) is an oncogenic virus that causes serious economic losses in the poultry industry; unfortunately, there is no effective vaccine against ALV-J. DNA methylation plays a crucial role in several biological processes, and an increasing number of diseases have been proven to be related to alterations in DNA methylation. In this study, we screened ALV-J-positive and -negative chickens. Subsequently, we generated and provided the genome-wide gene expression and DNA methylation profiles by MeDIP-seq and RNA-seq of ALV-J-positive and -negative chicken samples; 8,304 differentially methylated regions (DMRs) were identified by MeDIP-seq analysis (p ≤ 0.005) and 515 differentially expressed genes were identified by RNA-seq analysis (p ≤ 0.05). As a result of an integration analysis, we screened six candidate genes to identify ALV-J-negative chickens that possessed differential methylation in the promoter region. Furthermore, TGFB2 played an important role in tumorigenesis and cancer progression, which suggested TGFB2 may be an indicator for identifying ALV-J infections.
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影响因子：
48
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